BD Pharmingen™ Purified Mouse Anti-NF-ATc1

The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and  nterleukins including IL-2, -3, -4 and -5.  NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses.

7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region  between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.