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Cell Preparation and Separation Reagents
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Western blot analysis of NCK on HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 40000 dilution of anti-NCK antibody.
Immunofluorescent staining of HISM cells with anti-NCK antibody.
BD Transduction Laboratories™ Purified Mouse Anti-NCK
BD Transduction Laboratories™ Purified Mouse Anti-NCK
监管状态图例
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准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Dalva MB, Takasu MA, Lin MZ. EphB receptors interact with NMDA receptors and regulate excitatory synapse formation.. Cell. 2000; 103(6):945-956. (Clone-specific: Immunofluorescence). 查看参考
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McManus MJ, Boerner JL, Danielsen AJ, Wang Z, Matsumura F, Maihle NJ. An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex.. J Biol Chem. 2000; 275(45):35328-35334. (Clone-specific: Western blot). 查看参考
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Miyoshi-Akiyama T, Aleman LM, Smith JM, Adler CE, Mayer BJ. Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor.. Oncogene. 2001; 20(30):4058-4069. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Roche S, McGlade J, Jones M, Gish GD, Pawson T, Courtneidge SA. Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways.. EMBO J. 1996; 15(18):4940-4948. (Biology). 查看参考
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Schmitz U, Thommes K, Beier I. Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck.. J Biol Chem. 2001 ; 276(25):22003-22010. (Clone-specific: Western blot). 查看参考
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