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Western blot analysis of Moesin on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Moesin antibody.
Immunofluorescence staining of A498 cells (Human kidney carcinoma; ATCC HTB-44).
BD Transduction Laboratories™ Purified Mouse Anti-Moesin
BD Transduction Laboratories™ Purified Mouse Anti-Moesin
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Moesin belongs to a family of proteins that includes ezrin and radixin. These proteins are co-expressed in a variety of cells. They localize to the cytoskeleton and modify interactions between cytoskeletal and membrane proteins. There is approximately 70% homology between moesin and ezrin and 80% homology between moesin and radixin. Moesin is intracellular and contains multiple conserved domains that are putative phosphorylation sites. It has a calculated molecular weight of 67.8 kDa, but migrates in SDS-PAGE at approximately 78 kDa. This discrepancy is likely due to a charged alpha-helical region within the protein.
研发参考 (5)
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Lankes WT, Furthmayr H. Moesin: a member of the protein 4.1-talin-ezrin family of proteins. Proc Natl Acad Sci U S A. 1991; 88(19):8297-8301. (Biology). 查看参考
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Lankes WT, Schwartz-Albiez R, Furthmayr H. Cloning and sequencing of porcine moesin and radixin cDNA and identification of highly conserved domains. Biochim Biophys Acta. 1993; 1216(3):479-482. (Biology). 查看参考
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Parlato S, Giammarioli AM, Logozzi M, et al. CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway. EMBO J. 2000; 19(19):5123-5134. (Biology: Immunofluorescence, Western blot). 查看参考
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Poliak S, Matlis S, Ullmer C, Scherer SS, Peles E. Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells. J Cell Biol. 2002; 159(2):361-371. (Biology: Immunofluorescence). 查看参考
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Seveau S, Keller H, Maxfield FR, Piller F, Halbwachs-Mecarelli L. Neutrophil polarity and locomotion are associated with surface redistribution of leukosialin (CD43), an antiadhesive membrane molecule. Blood. 2000; 95(8):2462-2470. (Biology: Immunofluorescence, Western blot). 查看参考
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