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Western blot analysis of MEF2D on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2) . Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-MEF2D antibody.
Immunoflurorescence staining of Human Fibroblasts.
BD Transduction Laboratories™ Purified Mouse Anti-MEF2D
BD Transduction Laboratories™ Purified Mouse Anti-MEF2D
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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MEF2D is a member of the MEF2 (Myocyte-specific Enhancer-binding Factor 2) transcription factor family. Members of this family (MEF2A, B, C, & D) contain several characteristic motifs that include a MEF2 domain and a MADS box. MEF2 proteins bind the DNA sequence, 5'- (C/T)T(A/T)(A/T)AAATA(A/G)-3', and binding occurs primarily, although not exclusively, in muscle cells. MEF2D gene expression is ubiquitous and the mRNA is alternatively spliced. This results in multiple protein isoforms that heterodimerize with other family members. In addition to the MEF2 and MADS domains, MEF2D has a glutamine-/proline-rich region, amino acids 365-404, and a basic region, in the MADS domain near the N-terminus. The glutamine-/proline-rich region is involved in transactivation, while the basic region mediates sequence-specific DNA binding. MEF2D displays 75% similarity to hMEF2A and hMEF2C. Myogenin gene transcription requires MEF2D, indicating that MEF2D is involved in myogenesis and muscle cell differentiation. MEF2D has been reported to have a calculated molecular weight of 56 kD, but can be observed migrating in a range of 56-70 kD.
研发参考 (4)
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Breitbart RE, Liang CS, Smoot LB, Laheru DA, Mahdavi V, Nadal-Ginard B. A fourth human MEF2 transcription factor, hMEF2D, is an early marker of the myogenic lineage. Development. 1993; 118(4):1095-1106. (Biology). 查看参考
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Guttridge DC, Mayo MW, Madrid LV, Wang CY, Baldwin AS. NF-kappaB-induced loss of MyoD messenger RNA: possible role in muscle decay and cachexia. J Biol Chem. 2000; 298(5488):2363-2366. (Biology: Western blot). 查看参考
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Martin JF, Miano JM, Hustad CM, Copeland NG, Jenkins NA, Olson EN. A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing. Mol Cell Biol. 1994; 14(3):1647-1656. (Biology). 查看参考
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Mora S, Pessin JE. The MEF2A isoform is required for striated muscle-specific expression of the insulin-responsive GLUT4 glucose transporter. J Biol Chem. 2000; 275(21):16323-16328. (Biology: Immunoprecipitation). 查看参考
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