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- BD Rhapsody™ ATAC-Seq Assays
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Western blot analysis of LSF on a HCT-8 cell lysate (Human colorectal adenocarcinoma; ATCC CCL-244). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti- LSF antibody.
Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).
BD Transduction Laboratories™ Purified Mouse Anti-LSF
BD Transduction Laboratories™ Purified Mouse Anti-LSF
监管状态图例
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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LSF (αCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth.
This antibody is routinely tested by western blot analysis. Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (2)
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Shirra MK, Zhu Q, Huang HC, Pallas D, Hansen U. One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs. Mol Biol Cell. 1994; 14(8):5076-5087. (Biology). 查看参考
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Volker JL, Rameh LE, Zhu Q, DeCaprio J, Hansen U. Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity. Genes Dev. 1997; 11(46):1435-1446. (Biology). 查看参考
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