BD Transduction Laboratories™ Purified Mouse Anti-JBP1/Skb1Hs
克隆 32/JBP1 (RUO)
Western blot analysis of JBP1/SKB1HS on SW13 lysate. Lane 1: 1:500), lane 2: 1:1000, lane 3: 1:2000 of JBP1/SKB1HS.
PC12
Western blot analysis of JBP1/SKB1HS on SW13 lysate. Lane 1: 1:500), lane 2: 1:1000, lane 3: 1:2000 of JBP1/SKB1HS.
PC12
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
p21[Cdc42/Rac]-activated kinases (PAKs) bind and are activated by the small GTPases, Cdc42 and Rac. In yeast, the interaction of the PAK, Shk1, with Skb1 (Shk1 kinase binding protein 1) is important in the regulation of cell morphology and mitosis. Skb1Hs, the human homolog of Skb1, restores growth rates in yeast cells deficient for Skb1. Skb1Hs has also been identified as a 72 kDa pICln-binding protein (IBP72). pICln is thought to be a cytosolic regulator of a swelling-induced chloride channel and interactions with Skb1Hs suggest a link with PAKs, which are involved in cytoskeletal rearrangement. An additional line of study has identified Skb1Hs as a Jak binding protein (JBP1) via its interactions with Jak1-3 and Tyk2. This study also identified Skb1Hs as a methyltransferase that interacts with multiple proteins which possibly serve as physiological targets. Skb1Hs is expressed in a wide range of tissues and is thought to form homodimeric or multimeric complexes. Thus, Skb1Hs (IBP72, JBP1) is a multifunctional protein that may participate in such diverse processes as transcriptional and translational regulation, cytoskeletal rearrangement, and any of a number of signaling pathways.
研发参考 (3)
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Gilbreth M, Yang P, Bartholomeusz G, et al. Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. Proc Natl Acad Sci U S A. 1998; 95(25):14781-14786. (Biology). 查看参考
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Krapivinsky G, Pu W, Wickman K, Krapivinsky L, Clapham DE. pICln binds to a mammalian homolog of a yeast protein involved in regulation of cell morphology. J Biol Chem. 1998; 273(18):10811-10814. (Biology). 查看参考
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Pollack BP, Kotenko SV, He W, Izotova LS, Barnoski BL, Pestka S. The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity. J Biol Chem. 1999; 274(44):31531-31542. (Biology). 查看参考
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