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Western blot analysis of Ku80 on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human Ku80 antibody.
Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).
BD Transduction Laboratories™ Purified Mouse Anti-Human Ku80
BD Transduction Laboratories™ Purified Mouse Anti-Human Ku80
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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DNA-dependent protein kinase (DNA-PK) is a trimeric enzyme that contains a catalytic subunit of 350 kDa (DNA-PKcs) and a heterodimeric regulatory subunit of 70 kDa (Ku70) and 86 kDa (Ku80). DNA-PKcs is inactive alone and depends on the regulatory subunit for subcellular localization and kinase activity. The DNA-PKcs/Ku70/Ku80 complex is involved in V(D)J recombination and DNA double-stranded break repair. Ku70 and Ku80 are abundant nuclear DNA-binding proteins. Besides functioning in a complex with DNA-PKcs, Ku proteins may act in multiple cellular processes including transcriptional regulation, ATPase and helicase activity, alteration in chromatin structure, cell cycle regulation, and maintenance of telomere length. In rat fibroblasts, Ku80 overexpression leads to hypermethylation and silencing of metallothionein gene expression. Ku70-/- and Ku80-/- cells are less resistant to anticancer drug-induced apoptosis, suggesting a role for Ku proteins in the prevention of apoptotic signaling. Thus, Ku70 and Ku80 may have multiple roles during DNA transcription, repair, and maintenance depending on the protein interactions that are involved.
研发参考 (3)
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Kim SH, Kim D, Han JS, et al. Ku autoantigen affects the susceptibility to anticancer drugs. Cancer Res. 1999; 59(16):4012-4017. (Biology). 查看参考
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Majumder S, Ghoshal K, Li Z, Jacob ST. Hypermethylation of metallothionein-I promoter and suppression of its induction in cell lines overexpressing the large subunit of Ku protein. J Biol Chem. 1999; 274(40):28584-28589. (Biology). 查看参考
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Smith GC, Jackson SP. The DNA-dependent protein kinase.. Genes Dev. 1999; 13(8):916-34. (Biology). 查看参考
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