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Purified Mouse Anti-Human Btk
商品详情
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human N-Terminal Btk aa. 2-172 Recombinant Protein
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
77 kDa
250 µg/ml
AB_398428
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611116 Rev. 1
抗体详情
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53/BTK

Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling.  It is a member of the Tec family of kinases which includes Tec and Itk.  This family is similar to the src family of tyrosine kinases. However, Tec family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain.  The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551.  This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain.  Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate.  The PH domain is essential for proper activation and function of Btk.  A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

611116 Rev. 1
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611116 Rev.1
报价单和参考
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View product citations for antibody "611116" on CiteAb

研发参考 (5)

  1. Aoki Y, Isselbacher KJ, Pillai S. Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells. Proc Natl Acad Sci U S A. 1994; 91(22):10606-10609. (Biology). 查看参考
  2. Shahan TA, Sorenson WG, Simpson J, Kefalides NA, Lewis DM. Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages. J Biol Chem. 2000; 275(14):10175-10181. (Clone-specific: Immunoprecipitation, In vitro kinase assay, Western blot). 查看参考
  3. Sideras P, Müller S, Shiels H. Genomic organization of mouse and human Bruton's agammaglobulinemia tyrosine kinase (Btk) loci. J Immunol. 1994; 153(12):5607-5617. (Biology). 查看参考
  4. Vetrie D, Vorechovský I, Sideras P. The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases. Nature. 1993; 361(6409):226-233. (Biology). 查看参考
  5. Yang W, Malek SN, Desiderio S. An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo. J Biol Chem. 1995; 270(35):20832-20840. (Biology). 查看参考
查看所有文件 (5) 查看更少内容
611116 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.