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Cell Preparation and Separation Reagents
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Western blot analysis of Hsp90 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Hsp90 antibody.
Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).
BD Transduction Laboratories™ Purified Mouse Anti-Hsp90
BD Transduction Laboratories™ Purified Mouse Anti-Hsp90
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The 90 kDa heat shock protein (Hsp90), a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 interacts with a number of signaling molecules, other heat shock proteins, and cytoskeletal proteins. However, the function of Hsp90 in these complexes remains unclear. It is thought that Hsp90 plays a common role in a number of diverse signaling systems due to its physical association with v-Src family tyrosine kinases, steroid hormone receptors, the Raf serine/threonine kinase, the basic-helix-loop-helix dioxin receptor, and the βγ subunits of G proteins.
研发参考 (5)
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Brouet A, Sonveaux P, Dessy C, Balligand JL, Feron O. Hsp90 ensures the transition from the early Ca2+-dependent to the late phosphorylation-dependent activation of the endothelial nitric-oxide synthase in vascular endothelial growth factor-exposed endothelial cells. J Biol Chem. 2001; 276(35):32663-32669. (Biology: Immunoprecipitation, Western blot). 查看参考
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Miyamoto A, Nakayama K, Imaki H. Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta. Nature. 2002; 416(6883):865-869. (Biology: Western blot). 查看参考
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Murata T, Sato K, Hori M, Ozaki H, Karaki H. Decreased endothelial nitric-oxide synthase (eNOS) activity resulting from abnormal interaction between eNOS and its regulatory proteins in hypoxia-induced pulmonary hypertension. J Biol Chem. 2002; 277(46):44085-44092. (Biology: Western blot). 查看参考
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Pritchard KA Jr, Ackerman AW. Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase. J Biol Chem. 2001; 276(21):17621-17624. (Biology: Western blot). 查看参考
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Rebbe NF, Ware J, Bertina RM, Modrich P, Stafford DW. Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family. Gene. 1987; 53(2-3):235-245. (Biology). 查看参考
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