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Western blot analysis of GST-π on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- GST-π antibody.
Immunofluorescence staining of human fibroblasts.
BD Transduction Laboratories™ Purified Mouse Anti- GST-π
BD Transduction Laboratories™ Purified Mouse Anti- GST-π
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Glutathione-S-Transferases (GSTs) are a family of dimeric proteins that catalyze the S-conjugation of glutathione with many compounds for detoxification. The GSTs are categorized into four classes based on their biochemical and structural properties: α, µ, π, and θ. GST-π is of particular interest because it is over-expressed in many tumors, but is either absent or expressed at low levels in the corresponding normal tissues. This high expression of GST-π is associated with malignant transformation and correlates with a decrease in GST activity. Three different GST-π proteins have been isolated and designated as hGSTP1*A, hGSTP1*B, and hGSTP1*C. These isoforms are nearly identical, varying by only one to two amino acids.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (3)
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Ali-Osman F, Akande O, Antoun G, Mao JX, Buolamwini J. Molecular cloning, characterization, and expression in Escherichia coli of full-length cDNAs of three human glutathione S-transferase Pi gene variants. Evidence for differential catalytic activity of the encoded proteins. J Biol Chem. 1987; 272(15):10004-10012. (Biology). 查看参考
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Kano T, Sakai M, Muramatsu M. Structure and expression of a human class pi glutathione S-transferase messenger RNA. Cancer Res. 1987; 47(21):5626-5630. (Biology). 查看参考
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Zhou T, Evans AA, London WT. Glutathione S-transferase expression in hepatitis B virus-associated human hepatocellular carcinogenesis. Cancer Res. 1997; 57(13):2749-2753. (Biology). 查看参考
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