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Western blot analysis of gp91[phox] on mouse macrophage lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of gp91[phox] antibody.
Immunofluorescent staining of mouse macrophage cells with gp91[phox] antibody.
BD Transduction Laboratories™ Purified Mouse Anti-gp91[phox]
BD Transduction Laboratories™ Purified Mouse Anti-gp91[phox]
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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Although dormant in resting granulocytes, macrophages, and B lymphocytes, the neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. It is an integral membrane cytochrome, b558, which consists of two subunits, gp91[phox] and p21[phox]. Upon stimulation, cytochrome b558 forms a complex with cytosolic proteins, p67[phox], p47[phox], p40[phox], and rac2 and produces superoxide anions in a NADPH-dependent manner. In chronic granulomatous disease (CGD), severe recurrent bacterial and fungal infections result from defective NADPH-oxidase activity. The majority of CGD cases are caused by a defective gp91[phox] gene. gp91[phox], implicated as a docking site for p47[phox], is membrane glycoprotein with multiple N-terminal hydrophobic domains and a hydrophilic C-terminus. The expression of gp91[phox] is restricted to terminally differentiated phagocytes and B lymphocytes. This cell type- and developmental stage-specific expression may be controlled by transcriptional activators, such as PU.1 and YY1, and transcriptional repressors, such as CCAAT displacement protein (CDP). Endogenous mouse gp91phox shows 58 kDa band, instead of 91 kDa observed in human. Both mouse and human deglycosylated gp91 are 54 kDa. This difference may be due to less glycosylation sites in the mouse sequence.
研发参考 (5)
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Agarwal D, Dange R, Vila J, Otamendi A, Francis J. Detraining differentially preserved beneficial effects of exercise on hypertension: effects on blood pressure, cardiac function, brain inflammatory cytokines and oxidative stress. PLoS ONE. 2012; 7(12):e52569. (Clone-specific: Western blot). 查看参考
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Jacobsen BM, Skalnik DG. YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91(phox) promoter. J Biol Chem. 1999; 274(42):29984-29993. (Biology). 查看参考
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Suzuki S, Kumatori A, Haagen IA. PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes. Proc Natl Acad Sci U S A. 1998; 95(11):6085-6090. (Biology). 查看参考
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Yu L, Quinn MT, Cross AR, Dinauer MC. Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase. Proc Natl Acad Sci U S A. 1998; 95(14):7993-7998. (Biology). 查看参考
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Zhen L, Yu L, Dinauer MC. Probing the role of the carboxyl terminus of the gp91phox subunit of neutrophil flavocytochrome b558 using site-directed mutagenesis. J Biol Chem. 1998; 273(11):6575-6581. (Biology). 查看参考
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