BD Transduction Laboratories™ Purified Mouse Anti-Glutamine Synthetase
克隆 6/Glutamine Synthetase
(RUO)
Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody. Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visible staining of astrocytes in the section. Magnification: 40X. Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™ 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton™ X-100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).
Western blot analysis of glutamine synthetase on a rat cerebrum lysate (left). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti- glutamine synthetase antibody. Glutamine synthetase staining on a rat cerebrum section (center). Section prepared during antibody development was formalin fixed and paraffin embedded without citrate buffer pretreatment. Note visible staining of astrocytes in the section. Magnification: 40X. Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Glutamine Synthetase antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™ 855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software. This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton™ X-100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).
商品详情
BD Transduction Laboratories™
Glutamate-Ammonia Ligase; GLUL; GLNS
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2a
Human Glutamine Synthetase aa. 1-373
Western blot (Routinely Tested), Bioimaging, Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_397879
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
准备和存储
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
推荐的实验流程
For Western blot and IHC: Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp
For Bioimaging: Please refer to http://www.bdbiosciences.com/resources/cellularimaging/index.jsp
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Triton is a trademark of the Dow Chemical Company.
610517 Rev. 2
抗体详情
6/Glutamine Synthetase
Glutamine synthetase catalyzes the amination of glutamic acid to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
610517 Rev. 2
格式详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610517 Rev.2
报价单和参考
研发参考 (5)
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Fei ZL, D'Ambrosio C, Li S, Surmacz E, Baserga R. Association of insulin receptor substrate 1 with simian virus 40 large T antigen. Mol Cell Biol. 1995; 15(8):4232-4239. (Biology: Immunoprecipitation). 查看参考
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Kentroti S, Baker R, Lee K, Bruce C, Vernadakis A. Platelet-activating factor increases glutamine synthetase activity in early and late passage C-6 glioma cells. J Neurosci Res. 1991; 28(4):497-506. (Biology). 查看参考
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Kronfeld I, Kazimirsky G, Lorenzo PS, Garfield SH, Blumberg PM, Brodie C. Phosphorylation of protein kinase Cdelta on distinct tyrosine residues regulates specific cellular functions. J Biol Chem. 2000; 275(45):35491-35498. (Biology: Western blot). 查看参考
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Labow BI, Souba WW, Abcouwer SF. Glutamine synthetase expression in muscle is regulated by transcriptional and posttranscriptional mechanisms. Am J Physiol. 1999; 276(6):E1136-E1145. (Biology: Western blot). 查看参考
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Vardimon L, Fox LE, Cohen-Kupiec R, Degenstein L, Moscona AA. Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase. Mol Cell Biol. 1991; 11(10):5275-5284. (Biology). 查看参考
610517 Rev. 2
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
