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      Purified Mouse Anti-Eps8
      Purified Mouse Anti-Eps8
      Western blot analysis of Eps8 on a lysate from mouse macrophages (RAW 264.7) treated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours.  Lane 1:1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Eps8 antibody.
      Purified Mouse Anti-Eps8
      Immunofluorescence staining of rabbit cerebellum.
      Purified Mouse Anti-Eps8 Purified Mouse Anti-Eps8
      Western blot analysis of Eps8 on a lysate from mouse macrophages (RAW 264.7) treated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours.  Lane 1:1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Eps8 antibody.
      Immunofluorescence staining of rabbit cerebellum.
      商品详情
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      BD Transduction Laboratories™
      Mouse (QC Testing), Human,Rat,Dog (Tested in Development)
      Mouse IgG1
      Mouse Eps8 aa. 628-821
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      97 kDa
      250 µg/ml
      AB_397544
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610143 Rev. 2
      抗体详情
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      15/Eps8

      The p97 [Eps8] protein, a substrate for EGF-R tyrosine kinase, contains an SH3 domain, but lacks a functional SH2 domain. Antibodies to Eps8 recognize the 97 kDa protein and a less abundant 68 kDa protein. Both forms are tyrosine-phosphorylated following treatment of cells with EGF. It is likely that p68 [Eps8] is synthesized from an alternatively spliced mRNA since two major Eps8-specific mRNAs are detected by Northern analysis. Co-immunoprecipitation studies have demonstrated a physical association between the Eps8 protein and the EGF-R both in vivo and in vitro. For many EGF-R substrates, this interaction is mediated through an SH2 domain of the substrate. Since Eps8 lacks a well defined SH2 domain and a fusion protein containing the SH2-like region of Eps8 could not bind EGF-R, the mechanism of Eps8-EGF-R association remains unclear. Overexpression of Eps8 in fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, suggesting that Eps8 has a role in the modulation of EGF-R function.

      610143 Rev. 2
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610143 Rev.2
      报价单和参考
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      View product citations for antibody "610143" on CiteAb

      研发参考 (4)

      1. Burke P, Schooler K, Wiley HS. Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking. Mol Cell Biol. 2001; 12(6):1897-1910. (Biology: Western blot). 查看参考
      2. Fazioli F, Minichiello L, Matoska V. Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals. Science. 1993; 12(10):3799-3808. (Biology). 查看参考
      3. Miyamoto S, Teramoto H, Gutkind JS, Yamada KM. Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors. J Cell Biol. 1996; 135(6 pt 1):1633-1642. (Biology: Western blot). 查看参考
      4. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). 查看参考
      查看所有文件 (4) 查看更少内容
      610143 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.