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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Western blot analysis of EB1 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- EB1 antibody.
Immunofluorescence staining of human intestinal smooth muscle cells (HISM).
BD Transduction Laboratories™ Purified Mouse Anti-EB1
BD Transduction Laboratories™ Purified Mouse Anti-EB1
监管状态图例
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准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
配套商品
Familial adenomatous polyposis is an autosomal inherited disease characterized by mutations in the APC (adenomatous polyposis coli) gene. Individuals with this disease are predisposed to colorectal polyps and cancer. It has been proposed that disruption of the APC gene plays a role in the disease. APC is a large coiled-coil protein that interacts, via its carboxy terminus, with catenins and with the protein, EB1. This 30 kDa protein was discovered as a result of its strong binding to the C-terminal domain of APC. Like the APC-binding proteins β-Catenin and pp120Cas, EB1 may play an essential role in modulating APC activity and tumorigenesis.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Askham JM, Vaughan KT, Goodson HV, Morrison EE. Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Mol Biol Cell. 2002; 13(10):3627-3645. (Biology: Immunofluorescence, Western blot). 查看参考
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Etienne-Manneville S, Hall A. Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity. Nature. 2003; 421(6924):753-756. (Biology: Immunofluorescence). 查看参考
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Komarova YA, Akhmanova AS, Kojima S, Galjart N, Borisy GG. Cytoplasmic linker proteins promote microtubule rescue in vivo. J Cell Biol. 2002; 159(4):589-599. (Biology: Immunofluorescence, Western blot). 查看参考
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Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Western blot). 查看参考
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Su SL, Huang IP, Fair WR, Powell CT, Heston WD. Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression. Cancer Res. 1995; 55(7):2972-2977. (Biology). 查看参考
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