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Western blot analysis of DLP1 on a rat cerebrum lysate. 1 µg/mL (lane 1), 0.5 µg/mL (lane 2) and 0.25 µg/mL (lane 3) of the mouse anti-DLP1 antibody.
Immunofluorescence staining of human endothelial cells.
BD Transduction Laboratories™ Purified Mouse Anti-DLP1
BD Transduction Laboratories™ Purified Mouse Anti-DLP1
监管状态图例
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Dynamins, phosphoproteins with intrinsic GTPase activities, play an important role during early endocytosis. The GTPase activity is stimulated in vitro by microtubules, SH3 domain containing-proteins, and Protein Kinase (PKC)- mediated phosphorylation. Although highly related, dynamins are differentially expressed in an organism. For instance, dynamin I is found only in the nervous system, dynamin II is ubiquitously expressed, and dynamin III is located in lung, testis and brain. Due to four alternative splicing products for each of the dynamins, the dynamin family contains at least 12 different members. The Dynamin Like Protein-1 (DLP1) is a new member of the dynamin family of proteins. DLP1 (also known as DVLP) is widely expressed as two alternatively spliced products, each with different tissue expression patterns. The larger product is about 84 kDa and reportedly found only in the brain, whereas the 79 kDa product is ubiquitously expressed. Unlike dynamins I-III which co-localize with endocytotic vesicles, DLP1 is found aligned with microtubules and with the endoplasmic reticulum, suggesting a secretory function for this novel protein.
研发参考 (6)
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Bossya B, Bossy-Wetzela E, Petrillia A, et al. S-Nitrosylation of DRP1 Does Not Affect Enzymatic Activity and is Not Specific to Alzheimer’s Disease. 2010; 20:S513-S526. (Biology: Western blot). 查看参考
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Duvezin-Caubet S, Reichert AS, Jagasia R, et al. Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem. 2006; 281(49):37972-37979. (Biology: Western blot). 查看参考
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Estaquier J, Arnoult D. Inhibiting Drp1-mediated mitochondrial fission selectively prevents the release of cytochrome c during apoptosis. Cell Death Differ. 2007; 14(6):1086-1094. (Biology: Western blot). 查看参考
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Jofuku A, Ishihara N, Mihara K. Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein. Biochem Biophys Res Commun. 2005; 333(2):650-659. (Biology: Western blot). 查看参考
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Karbowski M, Neutzner A, Youle RJ. The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division. J Cell Biol. 2007; 178(1):71-84. (Biology: Immunofluorescence, Western blot). 查看参考
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Yoon Y, Pitts KR, Dahan S, McNiven MA. A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells. J Cell Biol. 1998; 140(4):779-793. (Biology). 查看参考
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