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Purified Mouse Anti-Cyclin D3
Purified Mouse Anti-Cyclin D3

Western blot analysis of Cyclin D3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Cyclin D3 antibody.

Purified Mouse Anti-Cyclin D3

Immunofluorescent staining of Human Endothelial cells.

Western blot analysis of Cyclin D3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Cyclin D3 antibody.

Immunofluorescent staining of Human Endothelial cells.

商品详情
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG2b
Human Cyclin D3 aa. 127-292
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
33 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610279 Rev. 2
抗体详情
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1/Cyclin D3

Several classes of cyclins (A-E) have been described. These proteins act as regulatory subunits for cyclin-dependent kinases (cdks). The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. There are at least 3 different D-type cyclins whose relative levels vary among cell types. Cyclin D3 is known to associate with Cdk5 and weakly with Cdk2. Cdk4 is preferentially bound by Cyclin D1. However, in T cells which do not express Cyclin D1, Cdk4 associates with Cyclins D2 and D3. While bound to cdks, the D-type cyclins also associate with the polymerase-δ subunit, PCNA. Cyclin D3 has also been shown to bind pRb in vitro. This suggests that pRb may be an in vivo substrate of D-type cyclins.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610279 Rev. 2
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610279 Rev.2
报价单和参考
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研发参考 (5)

  1. Bagui TK, Jackson RJ, Agrawal D, Pledger WJ. Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1). Mol Cell Biol. 2000; 20(23):8748-8757. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
  2. Haller K, Wu Y, Derow E, Schmitt I, Jeang KT, Grassmann R. Physical interaction of human T-cell leukemia virus type 1 Tax with cyclin-dependent kinase 4 stimulates the phosphorylation of retinoblastoma protein. Mol Cell Biol. 2002; 22(10):3327-3338. (Clone-specific: Western blot). 查看参考
  3. Motokura T, Keyomarsi K, Kronenberg HM, Arnold A. Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene. J Biol Chem. 1992; 267(28):20412-20415. (Biology). 查看参考
  4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence). 查看参考
  5. Xiong Y, Menninger J, Beach D, Ward DC. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics. 1992; 13(3):575-584. (Biology). 查看参考
查看所有文件 (5) 查看更少内容
610279 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.