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Purified Mouse Anti- CUL-3
Purified Mouse Anti- CUL-3
Western blot analysis of CUL-3 on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1:20,000, lane 3: 1:40,000 dilution of the mouse anti- CUL-3 antibody.
Purified Mouse Anti- CUL-3
Immunofluorescence staining of human endothelial cells.
Western blot analysis of CUL-3 on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1:20,000, lane 3: 1:40,000 dilution of the mouse anti- CUL-3 antibody.
Immunofluorescence staining of human endothelial cells.
商品详情
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BD Transduction Laboratories™
Rat (QC Testing), Human,Mouse,Dog,Chicken (Tested in Development)
Mouse IgG1
Human CUL-3 aa. 565-684
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
89 kDa
250 µg/ml
AB_399328
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611848 Rev. 1
抗体详情
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3/CUL-3

The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul proteins, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. The human Cul family of proteins, Cul-1, -2, -3, -4A, -4B, and -5, have also been implicated in oncogenic processes. Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome. Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas. Cul-3 is widely expressed in normal cells and tissues, and shows increased expression in colon cancer cells. Cul-3 can associate with cyclin E in mammalian cells, and overexpression of Cul-3 targets cyclin E for ubiquination, while deletion of Cul-3 in mice leads to increased cyclin E accumulation and abnormal regulation of S-phase. Thus, the Cul family of proteins may have roles in cell cycle regulation and tumoriogenesis.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611848 Rev. 1
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611848 Rev.1
报价单和参考
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研发参考 (3)

  1. Hori T, Osaka F, Chiba T, et al. Covalent modification of all members of human cullin family proteins by NEDD8. Oncogene. 1999; 18(48):6829-6834. (Biology). 查看参考
  2. Hori T, Osaka F, Chiba T. Covalent modification of all members of human cullin family proteins by NEDD8. Oncogene. 1999; 18(48):6829-6834. (Biology). 查看参考
  3. Singer JD, Gurian-West M, Clurman B, Roberts JM. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 1999; 13(18):2375-2387. (Biology). 查看参考
611848 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.