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Western blot analysis of c-Raf-1 on A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of c-Raf-1 antibody.
Immunofluorescent staining of A431 cells with c-Raf-1 antibody.
BD Transduction Laboratories™ Purified Mouse Anti-c-Raf
BD Transduction Laboratories™ Purified Mouse Anti-c-Raf
监管状态图例
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准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals. Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity. This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases. Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK). ERK then phosphorylates and activates Rsk. Raf activity can also be regulated independently of Ras. Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.
研发参考 (5)
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Bondzi C, Grant S, Krystal GW. A novel assay for the measurement of Raf-1 kinase activity. Oncogene. 2000; 19(43):5030-5033. (Clone-specific: Immunoprecipitation, In vitro kinase assay, Western blot). 查看参考
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Cheng JJ, Wung BS, Chao YJ, Wang DL. Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain. J Biol Chem. 2001; 276(33):31368-31375. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Coles LC, Shaw PE. PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway. Oncogene. 2002; 21(14):2236-2244. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Rapp UR. Role of Raf-1 serine/threonine protein kinase in growth factor signal transduction. Oncogene. 1991; 6(4):495-500. (Biology). 查看参考
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Rizzo MA, Kraft CA, Watkins SC, Levitan ES, Romero G. Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade. J Immunol. 2001; 276(37):34928-34933. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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