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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
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- BD® Single-Cell Multiplexing Kit
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Western blot analysis of BRUCE on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-BRUCE antibody.
Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).
BD Transduction Laboratories™ Purified Mouse Anti-BRUCE
BD Transduction Laboratories™ Purified Mouse Anti-BRUCE
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp
Clone 4/BRUCE has also been shown to work well for Western blot application on Hela lysate (Cat. No. 611449)
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Selective proteolysis is essential for the modulation of key cellular processes such as cell cycle progression. However, unlike other post-translational events, proteolysis is irreversible and therefore must occur in unidirectional cellular pathways. In eukaryotes, proteolysis is mediated primarily by the ubiquitin pathway. This pathway designates proteins for degradation by the proteasome, a multicatalytic protease complex. The ubiquitin pathway is a multistep system that tags proteins for degradation via the attachment of ubiquitin molecules to the target. This attachment is mediated by the ubiquitin activating/conjugating enzymes E1, E2, E3, and BRUCE. BRUCE (BIR Repeat containing Ubiquitin-Conjugating Enzyme) is a novel enzyme that associates with the Golgi and the vesicular system. It contains a UBC (ubiquitin conjugating enzyme) domain, which is essential for catalysis, and a BIR (baculovirus inhibitor of apoptosis repeat) motif. BIR motifs are also found within inhibitor of apoptosis proteins (IAP) and are critical for anti-apoptotic activity. Therefore, BRUCE may function to both mediate ubiquitin-dependent proteolysis and contribute to anti-apoptotic cellular pathways.
研发参考 (1)
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Hauser HP, Bardroff M, Pyrowolakis G, Jentsch S. A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors. J Cell Biol. 1998; 141(6):1415-1422. (Biology). 查看参考
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