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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
配套商品
β-Arrestins were discovered due to their ability to modulate interactions between the phosphorylated β2-Adrenergic receptors and G proteins. This modulation results in diminished β2-Adrenergic receptor function, also known as desensitization. Because arrestins are found at the synaptic terminals, they may provide a termination mechanism that allows the neurons to regain their original polarization and respond to a new neurotransmitter stimulus. The C-terminal region of arrestins is involved in selecting the phosphorylated and activated adrenergic receptors. The β-Arrestin1 gene encodes a protein of 418 amino acids with an approximate molecular weight of 55kDa. β-Arrestin1 protein is highly homologous to the 45kDa β-Arrestin2. Both proteins are widely expressed, but are especially abundant in the central nervous system.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (5)
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Attramadal H, Arriza JL, Aoki C, et al. Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family. J Biol Chem. 1992; 267(25):17882-17890. (Biology). 查看参考
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Dalle S, Imamura T, Rose DW, et al. Insulin induces heterologous desensitization of G-protein-coupled receptor and insulin-like growth factor I signaling by downregulating beta-arrestin-1. Mol Cell Biol. 2002; 22(17):6272-6285. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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DeFea KA, Zalevsky J, Thoma MS, Dery O, Mullins RD, Bunnett NW. beta-arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J Cell Biol. 2000; 148(6):1267-1281. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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Gurevich VV, Dion SB, Onorato JJ, et al. Arrestin interactions with G protein-coupled receptors. Direct binding studies of wild type and mutant arrestins with rhodopsin, beta 2-adrenergic, and m2 muscarinic cholinergic receptors. J Biol Chem. 1995; 270(2):720-731. (Biology). 查看参考
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Imamura T, Huang J, Dalle S, et al. beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport. J Biol Chem. 2001; 276(47):43663-43667. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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