Purified Mouse Anti-Annexin II

BD Transduction Laboratories™ Purified Mouse Anti-Annexin II

Name: Purified Mouse Anti-Annexin II
Brand: Purified Mouse Anti-Annexin II
SKU: 610068

克隆 5/Annexin II (RUO)

Western blot analysis of Annexin II on a A431 lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- Annexin II antibody.

Immunofluorescence staining of human fibroblasts.

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实验方案

科学资源

目录编号:610068

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目录价格:¥4,428.00

单位:EA (1 Each)

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商品详情

Brand: BD Transduction Laboratories™

Alternative Name: Lipocortin II, Calpactin I

Reactivity: Human (QC Testing), Mouse,Rat,Dog,Chicken (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human Annexin II aa. 123-328

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)

Target Molecular Weight: 36 kDa

Concentration: 250 µg/ml

RRID: AB_397480

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

数据表

技术数据表（TDS）

安全数据表

配套商品

HRP Goat Anti-Mouse Ig RUO

尺寸 1 mL 品类编号 554002

FITC Goat Anti-Mouse Ig RUO

尺寸 0.5 mg 品类编号 554001

610068 Rev. 1

抗体详情

5/Annexin II

Annexin II (p36), originally discovered as a substrate for the src oncogene, is one of the most studied members of the annexin family of calcium-dependent phospholipid-binding proteins. It exists in cells as either a monomer or a heterotetramer complexed with p11, an S100-related protein. In vitro, it has been found that unphosphorylated annexin II is capable of causing aggregation of chromaffin granules, and, upon phosphorylation, induces fusion of the granules' membranes. A similiar phenomenon may occur in the final steps of exocytosis after cytoplasmic annexin translocates to the subplasmalemmal region and is phosphorylated by protein kinase C. However, other studies refute annexin II's involvement in exocytosis, and a conclusive role for annexin II in membrane trafficking remains debated.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610068 Rev. 1

格式详情

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

格式: Purified

610068 Rev.1

报价单和参考

View Development References

研发参考 (5)

Chasserot-Golaz S, Vitale N, Sagot I, et al. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells. J Cell Biol. 1996; 133(6):1217-1236. (Biology). 查看参考
Choi KS, Fitzpatrick SL, Filipenko NR, et al. Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer. J Biol Chem. 2001; 276(27):25212-25221. (Biology: Immunohistochemistry). 查看参考
Hansen MD, Ehrlich JS, Nelson WJ. Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells. J Cell Biol. 2002; 277(47):45371-45376. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
Jacovina AT, Zhong F, Khazanova E, Lev E, Deora AB, Hajjar KA. Neuritogenesis and the nerve growth factor-induced differentiation of PC-12 cells requires annexin II-mediated plasmin generation. J Biol Chem. 2001; 276(52):49350-49358. (Biology: Immunofluorescence, Western blot). 查看参考
Kwon M, Caplan JF, Filipenko NR, et al. Identification of annexin II heterotetramer as a plasmin reductase. J Biol Chem. 2002; 277(13):10903-10911. (Biology: Flow cytometry). 查看参考

查看所有文件 (5)

610068 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.