The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.