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Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (γ, α, δ, or ε and β1, β2, β3, or β4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain ( σ1, σ2, σ3, or σ4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. Adaptin γ shows more homology with Adaptin α than with Adaptin β. The conserved regions between Adaptins γ and α could be important for binding to other components of the AP-1 and AP-2 complexes, respectively.
研发参考 (5)
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Eskelinen EL, Illert AL, Tanaka Y. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002; 13(9):3355-3368. (Clone-specific: Electron microscopy). 查看参考
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Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002; 13(11):4060-4073. (Clone-specific: Immunoprecipitation). 查看参考
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Nagahama M, Suzuki M, Hamada Y, et al. SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation. Mol Biol Cell. 2003; 14(1):262-273. (Clone-specific: Immunofluorescence). 查看参考
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Robinson MS. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus. J Cell Biol. 1990; 111(6 Pt 1):2319-2326. (Biology). 查看参考
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Wasiak S, Legendre-Guillemin V, Puertollano R, et al. Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics. J Cell Biol. 2002; 158(5):855-862. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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