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Alexa Fluor® 647 Rat Anti-Human AID
商品详情
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BD Pharmingen™
AID; ARP2; CDA2; HEL-S-284; HIGM2; Cytidine aminohydrolase
Human (QC Testing)
Rat IgG2b, κ
Human AID (185-198) Peptide
Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_2739355
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. An isotype control should be used at the same concentration as the antibody of interest.
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565785 Rev. 1
抗体详情
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EK2-5G9

The EK2-5G9 monoclonal antibody specifically binds to human Activation-induced cytidine deaminase (AICDA), also known as AID, or Single-stranded DNA cytosine deaminase. AID is an enzyme that preferentially acts upon certain DNA sequence motifs, such as those located in immunoglobulin variable regions, to induce cytidine-to-uracil mutations. AID-induced mutations initiate both somatic hypermutation (SHM) and class switch recombination (CSR) of the immunoglobulin genes in activated B cells during immune responses. While essential for immunoglobulin gene diversification, dysregulated AID activity can result in genomic instability and oncogenic transformation. Originally thought to be B-cell specific, AID now appears to be abnormally expressed in several epithelial cancers. AID may also play roles in DNA demethylation.

565785 Rev. 1
格式详情
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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Alexa Fluor™ 647
653 nm
669 nm
565785 Rev.1
报价单和参考
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View product citations for antibody "565785" on CiteAb

研发参考 (4)

  1. Dahlberg CI, He M, Visnes T, et al. A novel mouse model for the hyper-IgM syndrome: a spontaneous activation-induced cytidine deaminase mutation leading to complete loss of Ig class switching and reduced somatic hypermutation.. J Immunol. 2014; 193(9):4732-8. (Biology). 查看参考
  2. Greiner A, Tobollik S, Buettner M, et al. Differential expression of activation-induced cytidine deaminase (AID) in nodular lymphocyte-predominant and classical Hodgkin lymphoma.. J Pathol. 2005; 205(5):541-7. (Immunogen: ELISA, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). 查看参考
  3. Klemm L, Duy C, Iacobucci I, et al. The B cell mutator AID promotes B lymphoid blast crisis and drug resistance in chronic myeloid leukemia.. Cancer Cell. 2009; 16(3):232-45. (Biology). 查看参考
  4. Rebhandl S, Huemer M, Greil R, Geisberger R. AID/APOBEC deaminases and cancer.. Oncoscience. 2015; 2(4):320-33. (Biology). 查看参考
查看所有文件 (4) 查看更少内容
565785 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.