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准备和存储
推荐的实验流程
ELISA: Purified Mouse Anti-Human TNF antibody (Clone MAb1, Cat. No. 551220) is useful as a capture antibody for a sandwich ELISA for measuring human TNF. The purified MAb1 antibody can be paired with Biotin Mouse Anti-Human TNF mAb ( Clone MAb11, Cat. No. 554511) as the detection antibody, with recombinant human TNF protein (Cat. No. 554618) as the standard. For complex biological samples, such as serum or plasma, investigators are encouraged to use the BD OptEIA™ Human TNF ELISA Set (Cat. No. 555212) or BD OptEIA™ Human TNF ELISA Kit II (Cat. No. 550610).
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The MAb1 antibody reacts with human tumor necrosis factor (TNF, aka TNF-α) protein. TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer.
研发参考 (3)
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Danis VA, Franic GM, Rathjen DA, Brooks PM. Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes. Clin Exp Immunol. 1991; 85(1):143-150. (Clone-specific: ELISA). 查看参考
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Hogan MM, Vogel SN. Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins. J Immunol. 1988; 141(12):4196-4202. (Methodology). 查看参考
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Rathjen DA, Cowan K, Furphy LJ, Aston R. Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors. Mol Immunol. 1991; 28(1-2):79-86. (Clone-specific: ELISA). 查看参考
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