Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Website Feedback
      Alert Icon
      PE Mouse anti-Human HLA-A2
      HLA-A2-2.png

      Expression of PE anti-human HLA-A2 on lymphocytes from positive and negative donors

      Human lymphocytes from either a positive or a negative donor were stained with PE anti-human HLA-A2 (clone: BB7.2; Cat. No. 558570) and compared to an isotype control (Cat. No. 555743).  The overlay histograms show the HLA-A2 present on lymphocytes from the positive donor (right panel; solid line), but not on lymphocytes from a negative donor (left panel; solid line).  In both figures, the isotype control is represented by the dashed line.  Flow cytometry was performed on a BD FACSCalibur™ flow system.

      558570Image1.png

      Expression of PE anti-human HLA-A2 on lymphocytes from positive and negative donors

      Human lymphocytes from either a positive or a negative donor were stained with PE anti-human HLA-A2 (clone: BB7.2; Cat. No. 558570) and compared to an isotype control (Cat. No. 555743).  The overlay histograms show the HLA-A2 present on lymphocytes from the positive donor (right panel; solid line), but not on lymphocytes from a negative donor (left panel; solid line).  In both figures, the isotype control is represented by the dashed line.  Flow cytometry was performed on a BD FACSCalibur™ flow system.

      HLA-A2-2.png 558570Image1.png Red-Cap-Blue-Label.jpg

      Expression of PE anti-human HLA-A2 on lymphocytes from positive and negative donors

      Human lymphocytes from either a positive or a negative donor were stained with PE anti-human HLA-A2 (clone: BB7.2; Cat. No. 558570) and compared to an isotype control (Cat. No. 555743).  The overlay histograms show the HLA-A2 present on lymphocytes from the positive donor (right panel; solid line), but not on lymphocytes from a negative donor (left panel; solid line).  In both figures, the isotype control is represented by the dashed line.  Flow cytometry was performed on a BD FACSCalibur™ flow system.

      Expression of PE anti-human HLA-A2 on lymphocytes from positive and negative donors

      Human lymphocytes from either a positive or a negative donor were stained with PE anti-human HLA-A2 (clone: BB7.2; Cat. No. 558570) and compared to an isotype control (Cat. No. 555743).  The overlay histograms show the HLA-A2 present on lymphocytes from the positive donor (right panel; solid line), but not on lymphocytes from a negative donor (left panel; solid line).  In both figures, the isotype control is represented by the dashed line.  Flow cytometry was performed on a BD FACSCalibur™ flow system.

      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG2b, κ
      Papain-solubilized HLA-A2
      Flow cytometry (Routinely Tested), Blocking (Reported)
      20 µl
      AB_647220
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      558570 Rev. 2
      抗体详情
      Down Arrow Up Arrow
      BB7.2

      The monoclonal antibody BB7.2 specifically binds to the α subunit of the human leukocyte antigen-A2 (HLA-A2), a class I molecule of the major histocompatibility complex (MHC).  The MHC gene locus encodes a group of highly polymorphic, cell-surface proteins that play a broad role in the immune response to protein antigens. MHC molecules bind and present small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Human (human leukocyte antigen/HLA) MHC molecules are comprised of two major classes, MHC class I and class II.  Functionally, class I MHC molecules bind peptides derived from intracellular antigens (eg, viral and some bacterial antigens) which are specifically recognized by CD8+ T cells. Class II MHC molecules bind antigens derived from pathogens multiplying in intracellular vesicles and ingested extracellular bacteria, both of which are recognized by CD4+ T cells. TCR recognize processed peptides bound to the MHC as well as regions of the MHC molecule itself. CD4 and CD8 accessory molecules strengthen the formation of the TCR-MHC complex through their interaction with non-polymorphic regions of the MHC molecule.

      558570 Rev. 2
      格式详情
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      496 nm, 566 nm
      576 nm
      558570 Rev.2
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "558570" on CiteAb

      研发参考 (3)

      1. Bjorkman PJ, Saper MA, Samraoui B, Bennett WS, Strominger JL, Wiley DC. Structure of the human class I histocompatibility antigen, HLA-A2. Nature. 1987; 329(6139):506-512. (Biology). 查看参考
      2. Brenner MB, McLean J, Yang SY, van der Poel JJ, Pious D, Strominger JL. Clonal T lymphocyte recognition of the fine structure of the HLA-A2 molecule. J Immunol. 1985; 135(1):384-390. (Biology). 查看参考
      3. Brodsky FM, Parham P, Barnstable CJ, Crumpton MJ, Bodmer WF. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979; 47:3-6. (Biology). 查看参考
      558570 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.