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BD™ Cytometric Bead Array (CBA) Phospho JNK1/2 (T183/Y185) Flex Set
(RUO)Figure 1. Example BD CBA Phospho JNK1/2 (T183/Y185) Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.
Linearity: An activated cell lysate was serially diluted to determine the linearity of the assay.
Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Phospho JNK1/2 (T183/Y185) Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and two replicates of three different sample levels from four separate experiments (inter-assay).
BD™ Cytometric Bead Array (CBA) Phospho JNK1/2 (T183/Y185) Flex Set
BD™ Cytometric Bead Array (CBA) Phospho JNK1/2 (T183/Y185) Flex Set
BD™ Cytometric Bead Array (CBA) Phospho JNK1/2 (T183/Y185) Flex Set
Phospho JNK1/2 (T183/Y185) Flex Set
Regulatory Status Legend
未经BD明确书面授权,严禁使用未经许可的任何商品。
描述
The BD™ CBA Phospho JNK1/2 (T183/Y185) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat c-Jun N-terminal kinase1/2 (JNK1/2), that has been threonine-phosphorylated at Thr-183 and tyrosine-phosphorylated at Tyr-185 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho JNK1/2 (T183/Y185) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho JNK1/2 (T183/Y185) Flex Set. The biology and function of JNK1/2 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
准备和存储
The Phospho JNK1/2 (T183/Y185) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
推荐的实验流程
The BD CBA Phospho JNK1/2 (T183/Y185) Flex Set must be used in conjunction with a BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005, 100 tests, or 560006, 500 tests) and a flow cytometer. Detailed instructions on the use of this product can be found in the manual for the BD CBA Cell Signaling Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Phospho JNK1/2 (T183/Y185) Flex Set will be 1,000 Units/mL. An example standard curve is shown in Figure 1.
The BD CBA Phospho JNK1/2 (T183/Y185) Flex Set cannot be used in the same assay well with the following BD CBA Cell Signaling Flex Set reagents:
Flex Set Bead Position Catalog Number
BD CBA Phospho BLNK (Y84) Flex Set C9 560063
BD CBA Phospho Btk (Y551) Flex Set D5 560004
BD CBA Phospho eNOS (T495) Flex Set C7 560065
BD CBA Phospho Syk (Y352) Flex Set B9 560014
BD CBA Phospho ZAP70 (Y319) Flex Set B8 560011
The BD CBA Phospho JNK1/2 (T183/Y185) Flex Set should not be used in the same assay well with any non-BD CBA Cell Signaling Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein Flex Sets) nor with any BD CBA Total Protein Cell Signaling Flex Set reagents. For an updated assay compatibility chart for the BD CBA Cell Signaling Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.
Performance
Limit of Detection: The theoretical limit of detection is 0.38 Units/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 Units/mL, n=30) + 2 standard deviations.
商品通知
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Warning: CBA lyophilized standard contains 32.07% sodium dodecyl sulfate (w/w) and 0.01% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May be harmful if swallowed. Causes skin irritation. Causes serious eye irritation. May cause an allergic skin reaction.Precautionary statements: Wear protective gloves/eye protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Dispose of contents/container in accordance with local/regional/national/international regulations.
描述 | 数量/尺寸 | 零件号 | EntrezGene ID |
---|---|---|---|
Phospho JNK1/2 (T183/Y185) Capture Bead B5 | 1 Vial(s) (1 ea) | 51-9005195 | N/A |
Phospho JNK1/2 (T183/Y185) PE Detection Reagent | 1 Vial(s) (1 ea) | 51-9003901 | N/A |
Phospho JNK1/2 (T183/Y185) Standard | 1 Vial(s) (1 ea) | 51-9004902 | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.