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      Human Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

      BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

      (RUO)
      商品详情
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      BD™ Cytometric Bead Array (CBA)
      RUO
      AB_2868899


      描述

      The BD™ CBA Human Th1/Th2 Cytokine Kit (Catalog No. 550749) can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.

       Vial label Reagent Quantity
       A1 Human IL-2 Capture Beads 1 vial, 0.8 mL
       A2 Human IL-4 Capture Beads 1 vial, 0.8 mL
       A3 Human IL-5 Capture Beads 1 vial, 0.8 mL
       A4 Human IL-10 Capture Beads 1 vial, 0.8 mL
       A5 Human TNF Capture Beads 1 vial, 0.8 mL
       A6 Human IFN-γ Capture Beads 1 vial, 0.8 mL
       B Human Th1/Th2 PE Detection Reagent 1 vial, 4 mL
       C Human Th1/Th2 Cytokine Standards 2 vials, 0.2 mL lyophilized
       D Cytometer Setup Beads 1 vial, 1.5 mL
       E1 PE Positive Control Detector 1 vial, 0.5 mL
       E2 FITC Positive Control Detector 1 vial, 0.5 mL
       F Wash Buffer 1 bottle, 130 mL
       G Assay Diluent 1 bottle, 30 mL
       H Serum Enhancement Buffer 1 bottle, 10 mL

       

      550749 Rev. 1
      组件
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      描述 数量/尺寸 零件号 EntrezGene ID
      C) Human Th1/Th2 Cytokine Standards N/A 51-2428KC N/A
      E1) PE Positive Control Detector N/A 51-2429KC N/A
      B Human Th1/Th2 PE Detection Reagent N/A 51-2427KC N/A
      Human IFN-γ Capture Beads 1 Each (1 ea) 51-9005727 N/A
      Human TNF Capture Beads 1 Each (1 ea) 51-9005724 N/A
      Human IL-10 Capture Beads 80 Tests (1 ea) 51-9005740 N/A
      Human IL-5 Capture Beads 1 Each (1 ea) 51-9005718 N/A
      Human IL-4 Capture Beads 1 Each (1 ea) 51-9005715 N/A
      Human IL-2 Capture Beads 80 Tests (1 ea) 51-9005736 N/A
      D Cytometer Setup Beads 1 Each (1 ea) 51-9005731 N/A
      BD(tm) CBA Serum Enhancement Buffer (H) N/A 51-9002150 N/A
      G Assay Diluent 1 Each (1 ea) 51-2432KC N/A
      F Wash Buffer 1 Each (1 ea) 51-2431KC N/A
      E2 FITC Positive Control Detector 1 Each (1 ea) 51-9005734 N/A
      E1 PE Positive Control Detector 1 Each (1 ea) 51-9005732 N/A
      550749 Rev. 1
      报价单和参考
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      研发参考 (13)

      1. Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
      2. Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
      3. Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
      4. Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
      5. Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
      6. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
      7. Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
      8. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
      9. Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
      10. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). 查看参考
      11. McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). 查看参考
      12. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
      13. Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
      查看所有文件 (13) 查看更少内容
      550749 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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