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Purified Mouse Anti-Rat CD31
Purified Mouse Anti-Rat CD31

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Purified Mouse Anti-Rat CD31 (Cat. No. 555025; Right Panel), followed by Biotin Rat Anti-Mouse IgG1 (Cat. No. 553441) and PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Purified Mouse Anti-Rat CD31 (Cat. No. 555025; Right Panel), followed by Biotin Rat Anti-Mouse IgG1 (Cat. No. 553441) and PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

商品详情
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BD Pharmingen™
PECAM-1; Pecam1; Pecam
Rat (QC Testing), Pig (Tested in Development)
Mouse BALB/c IgG1, κ
Lewis Rat Microglia
Flow cytometry (Routinely Tested), Blocking, ELISA, Immunohistochemistry, Immunoprecipitation (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555025 Rev. 7
抗体详情
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TLD-3A12

The TLD-3A12 antibody specifically recognizes CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leucocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat. In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leucocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis. The TLD-3A12 mAb partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This mouse anti-rat CD31 antibody crossreacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively. A suspension of Lewis rat microglial cells was used as the the immunogen.

555025 Rev. 7
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555025 Rev.7
报价单和参考
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研发参考 (5)

  1. DeLisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997; 151(3):671-677. (Biology). 查看参考
  2. DeLisser HM, Newman PJ, Albelda SM. Molecular and functional aspects of PECAM-1/CD31. Immunol Today. 1994; 15(10):490-495. (Biology). 查看参考
  3. Famiglietti J, Sun J, DeLisser HM, Albelda SM. Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity. J Cell Biol. 1997; 138(6):1425-1435. (Biology). 查看参考
  4. Wakelin MW, Sanz MJ, Dewar A, et al. An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane. J Exp Med. 1996; 184(1):229-239. (Biology). 查看参考
  5. Williams KC, Zhao RW, Ueno K, Hickey WF. PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J Neurosci Res. 1996; 45(6):747-757. (Immunogen: Blocking, ELISA, Immunohistochemistry, Immunoprecipitation). 查看参考
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555025 Rev. 7

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.