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Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™)
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™)

Two color analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063/553064) and purified 2.4G2 mAb. The staining by 2.4G2 antibody was detected with FITC-conjugated mouse anti-rat lg, κ chain mAb MRK-1 (Cat. No. 553872). Right: BALB/c splenocytes were stained with FITC-conjugated rat anti-mouse CD90.2 (Thy-1.2) mAb 53-2.1 (Cat. No. 553003/553004) in the presence of purified 2.4G2 mAb (filled histogram) and without 2.4G2 mAb (open histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Two color analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining. Left: BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063/553064) and purified 2.4G2 mAb. The staining by 2.4G2 antibody was detected with FITC-conjugated mouse anti-rat lg, κ chain mAb MRK-1 (Cat. No. 553872). Right: BALB/c splenocytes were stained with FITC-conjugated rat anti-mouse CD90.2 (Thy-1.2) mAb 53-2.1 (Cat. No. 553003/553004) in the presence of purified 2.4G2 mAb (filled histogram) and without 2.4G2 mAb (open histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

商品详情
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BD Pharmingen™
FcγRIII/FcγRII; Fcgr3/Fcgr2
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Mouse BALB/c Macrophage J774
Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)
0.5 mg/ml
AB_394656
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推荐的实验流程

To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent.

        To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis:

1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4˚C for 5 minutes.

2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells).

3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG2b, κ).

For additional information on using Mouse BD Fc Block™, refer to our website protocol at http://www.bdbiosciences.com/pharmingen/protocols/Immunophenotyping.shtml

商品通知

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553141 Rev. 16
抗体详情
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2.4G2

The 2.4G2 antibody specifically recognizes a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII (CD16) and FcγII (CD32) Receptors. It has also been reported to bind the FcγI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, Receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553141 Rev. 16
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553141 Rev.16
报价单和参考
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研发参考 (19)

  1. Araujo-Jorge T, Rivera MT, el Bouhdidi A, Daeron M, Carlier Y. An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4G2) decreases acute Trypanosoma cruzi infection in mice. Infect Immun. 1993; 61(11):4925-4928. (Clone-specific: Blocking). 查看参考
  2. Benhamou M, Bonnerot C, Fridman WH, Daeron M. Molecular heterogeneity of murine mast cell Fc gamma receptors. J Immunol. 1990; 144(8):3071-3077. (Clone-specific: Immunoprecipitation). 查看参考
  3. Jensen WA, Marschner S, Ott VL, Cambier JC. FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization. Biochem Soc Trans. 2001; 29(6):840-846. (Clone-specific: Blocking). 查看参考
  4. Kaji K, Takeshita S, Miyake K, Takai T, Kudo A. Functional association of CD9 with the Fc gamma receptors in macrophages. J Immunol. 2001; 166(5):3256-3265. (Clone-specific: (Co)-stimulation). 查看参考
  5. Katz HR, Arm JP, Benson AC, Austen KF. Maturation-related changes in the expression of Fc gamma RII and Fc gamma RIII on mouse mast cells derived in vitro and in vivo. J Immunol. 1990; 145(10):3412-3417. (Clone-specific: Immunoprecipitation). 查看参考
  6. Kurlander RJ, Ellison DM, Hall J. The blockade of Fc receptor-mediated clearance of immune complexes in vivo by a monoclonal antibody (2.4G2) directed against Fc receptors on murine leukocytes. J Immunol. 1984; 133(2):855-862. (Clone-specific: Blocking). 查看参考
  7. Latour S, Bonnerot C, Fridman WH, Daeron M. Induction of tumor necrosis factor-alpha production by mast cells via Fc gamma R. Role of the Fc gamma RIII gamma subunit. J Immunol. 1992; 149(6):2155-2162. (Clone-specific: (Co)-stimulation). 查看参考
  8. Lewis VA, Koch T, Plutner H, Mellman I. A complementary DNA clone for a macrophage-lymphocyte Fc receptor. Nature. 1986; 324(6095):372-375. (Clone-specific). 查看参考
  9. Maeda K, Burton GF, Padgett DA, et al. Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII). J Immunol. 1992; 148(8):2340-2347. (Clone-specific: Immunohistochemistry). 查看参考
  10. Mellman IS, Unkeless JC. Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody. J Exp Med. 1980; 152(4):1048-1069. (Clone-specific: Immunoprecipitation). 查看参考
  11. Perussia B, Tutt MM, Qiu WQ, et al. Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene. J Exp Med. 1989; 170(1):73-86. (Clone-specific). 查看参考
  12. Ravetch JV, Luster AD, Weinshank R, et al. Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors. Science. 1986; 234(4777):718-725. (Clone-specific). 查看参考
  13. Rodewald HR, Awad K, Moingeon P, et al. Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-1092. (Biology). 查看参考
  14. Rodewald HR, Moingeon P, Lucich JL, Dosiou C, Lopez P, Reinherz EL. A population of early fetal thymocytes expressing Fc gamma RII/III contains precursors of T lymphocytes and natural killer cells. Cell. 1992; 69(1):139-150. (Clone-specific: Immunoprecipitation). 查看参考
  15. Takezawa R, Watanabe Y, Akaike T. Direct evidence of macrophage differentiation from bone marrow cells in the liver: a possible origin of Kupffer cells. J Biochem (Tokyo). 1995; 118(6):1175-1183. (Clone-specific). 查看参考
  16. Titus JA, Finkelman FD, Stephany DA, Jones JF, Segal DM. Quantitative analysis of Fc gamma receptors on murine spleen cell populations by using dual parameter flow cytometry. J Immunol. 1984; 133(2):556-561. (Biology). 查看参考
  17. Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979; 150(3):580-596. (Immunogen). 查看参考
  18. Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Clone-specific). 查看参考
  19. Witmer MD, Steinman RM. The anatomy of peripheral lymphoid organs with emphasis on accessory cells: light-microscopic immunocytochemical studies of mouse spleen, lymph node, and Peyer's patch. Am J Anat. 1984; 170(3):465-481. (Clone-specific: Immunohistochemistry). 查看参考
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553141 Rev. 16

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