Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      PE Rat Anti-Mouse CD201
      PE Rat Anti-Mouse CD201
      Flow cytometric analysis of CD201 expression on bEND.3 cells. Cells from the mouse bEnd.3 (Endothelioma, ATCC CRL-2299) cell line were stained with either PE Rat IgG2b κ Isotype Control (Cat. No. 555848; dotted line histogram) or PE Rat Anti-Mouse CD201 antibody (Cat. No. 566337; solid line histogram) at 0.5 μg/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histogram showing CD201 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      PE Rat Anti-Mouse CD201
      Flow cytometric analysis of CD201 expression on bEND.3 cells. Cells from the mouse bEnd.3 (Endothelioma, ATCC CRL-2299) cell line were stained with either PE Rat IgG2b κ Isotype Control (Cat. No. 555848; dotted line histogram) or PE Rat Anti-Mouse CD201 antibody (Cat. No. 566337; solid line histogram) at 0.5 μg/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histogram showing CD201 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Procr; Epcr; APC receptor; Ccca; Centrocyclin; Ccd41
      Mouse (QC Testing)
      Rat IgG2b
      Mouse EPCR Recombinant Protein
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2739694
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      566337 Rev. 1
      抗体详情
      Down Arrow Up Arrow
      1560

      The 1560 monoclonal antibody specifically binds to CD201 which is also known as Endothelial protein C receptor (EPCR) or Activated protein C receptor (APC receptor). CD201 is encoded by Procr (Protein C receptor, endothelial). CD201 is a ~25 kDa single-pass type I transmembrane glycoprotein that binds both protein C and activated protein C (APC) and augments the activation of protein C by the thrombin-thrombomodulin/CD141 complex. APC functions as a serine protease that controls thrombin IIa generation by inhibiting factors Va and VIIIa. CD201 thus plays an essential role in preventing blood coagulation which may lead to tissue damage during sepsis and inflammatory responses. Procr-gene deletion reportedly leads to embryonic lethality due to placental thrombosis. CD201 is expressed by endothelial cells with higher expression on large blood vessels. The 1560 monoclonal antibody can reportedly be used for the detection of bone marrow hematopoietic stem cells (HSC) which express high levels of CD201. A soluble form of CD201 which can bind protein C and APC has also been described.

              

      566337 Rev. 1
      格式详情
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      566337 Rev.1
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "566337" on CiteAb

      研发参考 (5)

      1. Balazs AB, Fabian AJ, Esmon CT, Mulligan RC. Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow.. Blood. 2006; 107(6):2317-21. (Clone-specific: Blocking, Flow cytometry, Fluorescence activated cell sorting, Functional assay). 查看参考
      2. Crawley JT, Gu JM, Ferrell G, Esmon CT. Distribution of endothelial cell protein C/activated protein C receptor (EPCR) during mouse embryo development.. Thromb Haemost. 2002; 88(2):259-66. (Immunogen: Immunohistochemistry). 查看参考
      3. Disse J, Petersen HH, Larsen KS, et al. The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors.. J Biol Chem. 2011; 286(7):5756-67. (Clone-specific: Functional assay). 查看参考
      4. Gu JM, Crawley JT, Ferrell G, et al. Disruption of the endothelial cell protein C receptor gene in mice causes placental thrombosis and early embryonic lethality.. J Biol Chem. 2002; 277(45):43335-43. (Clone-specific: Immunohistochemistry). 查看参考
      5. Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex.. Proc Natl Acad Sci USA. 1996; 93(19):10212-6. (Biology). 查看参考
      查看所有文件 (5) 查看更少内容
      566337 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.