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PE Mouse Anti-Human Integrin α1 (CD49a)
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Flow cytometric analysis of Integrin α1 (CD49a) expression on Human Leucocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human Integrin α1 (CD49a) antibody (Cat. No. 568716/568717; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of Integrin α1 (CD49a) [or Ig Isotype control staining] versus side light-scatter signals (SSC) was derived from gated events with the light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.

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568717
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¥2,330.00
EA (1 Each)
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BD Pharmingen™
ITGA1; CD49a; VLA-1; VLA1; integrin alpha-1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human HLA-DR CTL line
Flow cytometry (Routinely Tested)
5 µl
AB_3684492
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

推荐的实验流程

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

商品通知

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

数据表

568717 Rev. 1
抗体详情
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TS2/7

The TS2/7 monoclonal antibody specifically recognizes Integrin alpha 1 (Integrin α1) which is also known as CD49a (CD49 antigen-like family member A) and Very late antigen 1α subunit (VLA-1). Integrin α1 (CD49a) is an ~200 kDa single pass type I transmembrane glycoprotein that is encoded by ITGA1 (Integrin subunit alpha 1) which belongs to the Integrin alpha chain family. Integrin α1 (CD49a) associates with the integrin β1 chain (CD29) to form the Integrin α1β1 heterodimer (also known as CD49a/CD29 or the VLA-1 complex) that serves as a receptor for collagen and laminin-1. It is expressed on activated T cells, monocytes, neuronal cells, and smooth muscle cells. Integrin α1 (CD49a) reportedly plays a role in leucocyte migration into tissues and can costimulate T cell proliferation and cytokine production. It is also involved in cellular attachment during the development of both the central and peripheral nervous systems.

568717 Rev. 1
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
568717 Rev.1
报价单和参考
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研发参考 (5)

  1. Hemler ME, Sanchez-Madrid F, Flotte TJ, et al. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines.. J Immunol. 1984; 132(6):3011-8. (Clone-specific: Flow cytometry, Immunohistochemistry). 查看参考
  2. Marquardt N, Kekäläinen E, Chen P, et al. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.. Nat Commun. 2019; 10(1):3841. (Clone-specific: Flow cytometry). 查看参考
  3. Melssen MM, Olson W, Wages NA, et al. Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma.. Oncoimmunology. 7(10):e1490855. (Clone-specific: Flow cytometry). 查看参考
  4. Sanchez-Madrid F, Krensky AM, Ware CF, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.. Proc Natl Acad Sci U S A. 1982; 79(23):7489-93. (Immunogen: Western blot). 查看参考
  5. Zola H. CD49a. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:122.
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568717 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.