Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas α cell line. LEFT PANEL: Untransfected (dashed-line histogram) and human Glucagon-transfected (solid-line histogram) 293F cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and then stained with PE Mouse Anti-Glucagon (Cat. No. 565860). RIGHT PANEL: Alpha TC1-6 cells (ATCC CRL-2934) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and then stained with either PE Mouse IgG1 κ Isotype control (Cat. No. 554680, dashed-line histogram) or PE Mouse Anti-Glucagon (Cat. No. 565860, solid-line histogram). All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
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Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas α cell line. LEFT PANEL: Untransfected (dashed-line histogram) and human Glucagon-transfected (solid-line histogram) 293F cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and then stained with PE Mouse Anti-Glucagon (Cat. No. 565860). RIGHT PANEL: Alpha TC1-6 cells (ATCC CRL-2934) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were washed and then stained with either PE Mouse IgG1 κ Isotype control (Cat. No. 554680, dashed-line histogram) or PE Mouse Anti-Glucagon (Cat. No. 565860, solid-line histogram). All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
商品详情
BD Pharmingen™
GLP1; GLP2; GRPP; GCG
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse BALB/c IgG1, κ
Human glucagon Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2739382
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- An isotype control should be used at the same concentration as the antibody of interest.
配套商品
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
Stain Buffer (FBS) RUO
尺寸
500 mL
品类编号
554656
Fixation Buffer RUO
尺寸
100 mL
品类编号
554655
Perm Buffer III RUO
尺寸
125 mL
品类编号
558050
PE Mouse IgG1, κ Isotype Control RUO
尺寸
0.1 mg
品类编号
554680
565860 Rev. 1
抗体详情
U16-850
The U16-850 monoclonal antibody specifically binds to glucagon, a member of the secretin family of active peptides. Glucagon is an evolutionarily conserved peptide hormone that participates in the regulation of carbohydrate metabolism by counteracting the effects of insulin. Glucagon is produced by α cells in the islets of Langerhans of the pancreas. The CGC gene encodes the precursor molecule preproglucagon, which is cleaved to form proglucagon that is in turn cleaved to form at least four distinct peptides, including glucagon, in different tissues. Hypoglycemia causes the secretion of glucagon, which binds to the class B G-protein-coupled glucagon receptor that is mainly expressed in liver and kidney, causing reduced glycogenesis and glycolysis and increased glycogenolysis and gluconeogenesis. The expression of glucagon can be used to monitor the pancreatic differentiation of pluripotent stem cells.
565860 Rev. 1
格式详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565860 Rev.1
报价单和参考
研发参考 (6)
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Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R. Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.. Hum Mol Genet. 2014; 23(13):3432-44. (Biology). 查看参考
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D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology). 查看参考
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Jiang G, Zhang BB. Glucagon and regulation of glucose metabolism.. Am J Physiol Endocrinol Metab. 2003; 284(4):E671-8. (Biology). 查看参考
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Kelly OG, Chan MY, Martinson LA, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011; 29(8):750-756. (Biology). 查看参考
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Thomsen J, Kristiansen K, Brunfeldt K, Sundby F. The amino acid sequence of human glucagon.. FEBS Lett. 1972; 21(3):315-319. (Biology). 查看参考
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Witt S, Dietz H, Ziegler B, Keilacker H, Ziegler M. Production and use of monoclonal glucagon and insulin antibodies--reduction of pancreatic insulin in rats by treatment with complete Freund's adjuvant. Acta Histochem Suppl. 1988; 35:217-223. (Biology). 查看参考
565860 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
