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      BV421 Mouse Anti-Human HLA-DR
      BV421 Mouse Anti-Human HLA-DR
      Multiparameter flow cytometric analysis of HLA-DR expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human HLA-DR (Cat No. 752486; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS™ Lysing Solution 10X Concentrate (Cat No. 349202). A bivariate pseudocolor density plot showing the correlated expression of HLA-DR (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact Human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Mouse Anti-Human HLA-DR
      Multiparameter flow cytometric analysis of HLA-DR expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human HLA-DR (Cat No. 752486; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS™ Lysing Solution 10X Concentrate (Cat No. 349202). A bivariate pseudocolor density plot showing the correlated expression of HLA-DR (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact Human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Mouse Anti-Human HLA-DR BV421 Mouse Anti-Human HLA-DR
      Multiparameter flow cytometric analysis of HLA-DR expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human HLA-DR (Cat No. 752486; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS™ Lysing Solution 10X Concentrate (Cat No. 349202). A bivariate pseudocolor density plot showing the correlated expression of HLA-DR (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact Human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Multiparameter flow cytometric analysis of HLA-DR expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human HLA-DR (Cat No. 752486; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS™ Lysing Solution 10X Concentrate (Cat No. 349202). A bivariate pseudocolor density plot showing the correlated expression of HLA-DR (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact Human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD OptiBuild™
      HLA-DR alpha beta; HLA-DR alpha,beta; HLA-DR alpha-beta; HLA-DR alpha/beta; HLA-DRαβ
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      RPMI 8866 human lymphoblastoid cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      3122, 3123, 3125, 3126, 3127
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      6. Researchers should determine the optimal concentration of this reagent for their individual applications.
      7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      752486 Rev. 2
      抗体详情
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      L203.rMAb

      The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.

      752486 Rev. 2
      格式详情
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      752486 Rev.2
      报价单和参考
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      View product citations for antibody "752486" on CiteAb

      研发参考 (4)

      1. Beck B, Dörfel D, Lichtenegger FS, et al. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.. J Transl Med. 2011; 9:151. (Clone-specific: Flow cytometry). 查看参考
      2. Finn OJ, Levy R. Multiple HLA-DR antigens: detection with monoclonal antibodies and translation in vitro.. Proc Natl Acad Sci USA. 1982; 79(8):2658-62. (Clone-specific: Immunoprecipitation). 查看参考
      3. Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line.. J Immunol. 1980; 125(1):293-9. (Immunogen: Blocking, Immunoprecipitation, Radioimmunoassay). 查看参考
      4. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Clone-specific: Blocking, Functional assay, Inhibition). 查看参考
      查看所有文件 (4) 查看更少内容
      752486 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.