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568402_568403.png

Flow cytometric analysis of CD179b (λ5) expression on Human NALM6 cells.  Cells from the Human NALM6 (Acute lymphoblastic leukemia, ATCC® CRL-3273™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220). Cells were then stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438, dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human CD179b (λ5) antibody [Cat No. 568402/568403; solid line histogram) at 1 µg/test. The fluorescence histogram showing CD179b (λ5) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

568402_568403.png Blue-Cap-Violet-Label.png

Flow cytometric analysis of CD179b (λ5) expression on Human NALM6 cells.  Cells from the Human NALM6 (Acute lymphoblastic leukemia, ATCC® CRL-3273™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220). Cells were then stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat No. 562438, dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human CD179b (λ5) antibody [Cat No. 568402/568403; solid line histogram) at 1 µg/test. The fluorescence histogram showing CD179b (λ5) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

商品详情
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BD Horizon™
IGL1; IGLL1; VPREB2; ig lambda-5; immunoglobulin omega polypeptide chain; immunoglobulin-related 14.1 protein; lambda5
Human (QC Testing)
Mouse BALB/c IgG1, κ
Ltk– preBCR transfectants
Flow cytometry (Routinely Tested)
0.2 mg/ml
VII 70513
3543
AB_3684244
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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推荐的实验流程

   BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

   For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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商品通知

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. For U.S. patents that may apply, see bd.com/patents.
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568403 Rev. 1
抗体详情
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HSL11.rMAb

The HSL11.rMAb monoclonal antibody specifically recognizes CD179b, which is also known as Ig Lambda 5, λ5 or Ig omega. CD179b is a ~23 kDa protein within the immunoglobulin gene superfamily and is a component of the pre-B cell receptor complex (pre-BCR). The pre-BCR is a heterodimer comprised of an immunoglobulin heavy chain (IgH) and a surrogate light (SL) chain, which is composed of non-covalently linked CD179b (λ5) and CD179a (VpreB) proteins. The pre-BCR is expressed during the early stages of B lymphocytes development and its expression is restricted to pre-B cell stage of B cell development, whereas the expression of SL chain is restricted to both pro-B and pre-B cell stages of B cell development. Due to restricted lineage expression, CD179b (λ5) along with CD179a (VpreB) may serve as a useful marker of precursor B cells and can be utilized for immunophenotyping precursor B cell lymphoblastic lymphomas. The pre-BCR plays a role in signal transduction for cell proliferation and differentiation from the proB cell to the preB cell stage, allelic exclusion of IgH chain loci, and Ig light chain gene rearrangements. CD179b (λ5) is required for precursor B cell development and its deficiency could prevent the maturation and survival of the B cell precursors beyond the pre-B cell stage and can lead to B cell deficiency and agammaglobulinemia.

568403 Rev. 1
格式详情
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
568403 Rev.1
报价单和参考
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View product citations for antibody "568403" on CiteAb

研发参考 (4)

  1. Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
  2. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific). 查看参考
  3. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen). 查看参考
  4. Winkler TH, Mårtensson IL. The Role of the Pre-B Cell Receptor in B Cell Development, Repertoire Selection, and Tolerance. Front Immunol. 2018; 15(9):2423. (Biology). 查看参考
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568403 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.