The 190909 antibody specifically recognizes a common epitope on the extracellular domains of CD16 (also known as, Fc gamma RIII) and CD32 (Fc gamma RII). CD16 and CD32 serve as low affinity receptors for mouse IgG Fc constant regions and play roles in the activation or inhibition of phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), degranulation, and B cell proliferation. These receptors are variably expressed on B lymphocytes, natural killer (NK) cells, monocytes, macrophages, dendritic cells, Kupffer cells, granulocytes, mast cells, immature thymocytes, and some activated mature T lymphocytes. CD16 and CD32 are single-pass type I transmembrane glycoproteins with two extracellular Ig-like domains and belong to the Ig superfamily. Ligand-bound CD16 can associate with two disulfide-linked FcR-gamma subunits (FcεRIγ) that contain cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) which deliver activating signals intracellularly. CD32 has an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and functions as an inhibitory receptor.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).