Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      APC Rat Anti-Mouse CD205 (DEC-205)
      APC Rat Anti-Mouse CD205 (DEC-205)
      Two-color flow cytometric analysis of CD205 (DEC-205) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or APC Rat Anti-Mouse CD205 (DEC-205) antibody (Cat. No. 567500; Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 (DEC-205) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      APC Rat Anti-Mouse CD205 (DEC-205)
      Two-color flow cytometric analysis of CD205 (DEC-205) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or APC Rat Anti-Mouse CD205 (DEC-205) antibody (Cat. No. 567500; Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 (DEC-205) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      CD205; Cd205; DEC-205; DEC205; Ly-75; Ly75
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2a, κ
      Mouse lymph node stromal cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      6. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      7. An isotype control should be used at the same concentration as the antibody of interest.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      567500 Rev. 1
      抗体详情
      Down Arrow Up Arrow
      NLDC-145

      The NLDC-145 monoclonal antibody specifically recognizes CD205 which is also known as Dendritic and thymic Epithelial Cells, 205 kDa (DEC-205) or Lymphocyte antigen 75 (Ly-75). CD205 (DEC-205) is a ~205 kDa type 1 transmembrane glycoprotein that is encoded by Ly75 which belongs to the mannose receptor family within the C-type lectin superfamily. The extracellular region contains an N-terminal cysteine-rich domain followed by a fibronectin type II domain, 10 C-type lectin-like domains, a transmembrane region and cytoplasmic tail with an internalization sequence. CD205 (DEC-205) is highly expressed on both CD8+ and CD103+ dendritic cell subsets and Langerhans cells. It is variably expressed by some other dendritic cell types, Gr1+ bone marrow cells, B cells, T cells, thioglycolate-elicited macrophages, granulocytes, mucosal and thymic cortical epithelial cells, and brain endothelial cells. CD205 (DEC-205) serves as a pattern recognition receptor that functions as a recycling endocytic receptor. It participates in the cellular processing, presentation, and cross-presentation of foreign or self-antigens to initiate immunity or tolerance, respectively. CD205 (DEC-205) can reportedly serve as a promising target for immunotherapy including DC-targeted vaccination using anti-CD205 (DEC-205) monoclonal antibody conjugated with antigen for T cell activation.

      567500 Rev. 1
      格式详情
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      567500 Rev.1
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (12)

      1. East L, Isacke CM. The mannose receptor family.. Biochim Biophys Acta. 2002; 1572(2-3):364-86. (Clone-specific). 查看参考
      2. Hossain MK, Wall KA. Use of Dendritic Cell Receptors as Targets for Enhancing Anti-Cancer Immune Responses.. Cancers (Basel). 2019; 11(3):E418. (Biology). 查看参考
      3. Inaba K, Swiggard WJ, Inaba M, et al. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.. Cell Immunol. 1995; 163(1):148-56. (Clone-specific: Flow cytometry). 查看参考
      4. Jiang W, Swiggard WJ, Heufler C. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature. 1995; 375(6527):151-155. (Clone-specific). 查看参考
      5. Kraal G, Breel M, Janse M, Bruin G. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med. 1986; 163(4):981-997. (Immunogen). 查看参考
      6. Merad M, Sathe P, Helft J, Miller J, Mortha A. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting.. Annu Rev Immunol. 2013; 31:563-604. (Biology). 查看参考
      7. Steinman RM. Decisions about dendritic cells: past, present, and future.. Annu Rev Immunol. 2012; 30:1-22. (Clone-specific). 查看参考
      8. Steinman RM. Dendritic cells: understanding immunogenicity.. Eur J Immunol. 2007; 37 Suppl 1:S53-60. (Clone-specific). 查看参考
      9. Swiggard WJ, Mirza A, Nussenzweig MC, Steinman RM. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence.. Cell Immunol. 1995; 165(2):302-11. (Clone-specific: Immunoaffinity chromatography, Immunoprecipitation, Western blot). 查看参考
      10. Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). 查看参考
      11. Witmer-Pack MD, Swiggard WJ, Mirza A, Inaba K, Steinman RM. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression in situ in lymphoid and nonlymphoid tissues.. Cell Immunol. 1995; 163(1):157-62. (Clone-specific: Immunohistochemistry, Western blot). 查看参考
      12. Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Clone-specific: Flow cytometry). 查看参考
      查看所有文件 (12) 查看更少内容
      567500 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback