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Fixation/Permeabilization Solution Kit with BD GolgiPlug™

Fixation/Permeabilization Solution Kit with BD GolgiPlug™

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BD Cytofix/Cytoperm™ Plus


This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. This kit also provides an alternative protein transport inhibitor, BD GolgiPlug™ containing brefeldin A. Sufficient BD GolgiPlug reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10 cells/ml.

Components Quantity  Fixation/Permeabilization solution  125 mL  BD Perm/Wash™ buffer (10x)  100 mL  BD GolgiPlug protein transport inhibitor containing brefeldin A  1 mL

555028 Rev. 1
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描述 数量/尺寸 零件号 EntrezGene ID
Fixation/Permeabilization solution 125 mL (1 ea) 51-2090KZ N/A
BD Perm/Wash™ Buffer 100 mL (1 ea) 51-2091KZ N/A
Protein Transport Inhibitor (containing Brefeldin A) 1 Each (1 ea) 51-2301KZ N/A
555028 Rev. 1
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研发参考 (14)

  1. Alaverdi, N and JB Waters . Enhanced detection of antigens by flow cytometry with the BD Cytofix/Cytoperm™ intracellular staining kit. Hotlines. 3:6,7,15,16. (Biology).
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  3. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Biology). 查看参考
  4. Andersson, U and J Andersson. D Fradelizie and D Emelie, ed. Cytokine Producing Cells. Paris: INSERM; 1994:32-49.
  5. Assenmacher M, J Schmitz and A Radbruch. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of interleukin-10 in interferon-γ and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24:1097-1101. (Biology).
  6. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. (Biology). 查看参考
  7. Ferrick DA, Schrenzel MD, Mulvania T, Hsieh B, Ferlin WG, Lepper H. Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. Nature. 1995; 373(6511):255-257. (Biology). 查看参考
  8. Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. (Biology). 查看参考
  9. Litton M, Andersson J, Bjork L, Fehniger T, Ulfgren AK, Andersson U. Cytoplasmic cytokine staining in individual cells. In: Debets and Savelkoul, ed. Human Cytokine Protocols. Humana Press; 1996.
  10. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Biology). 查看参考
  11. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 查看参考
  12. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology). 查看参考
  13. Sander, B, J Andersson and U Andersson. Assessment of cytokines by immunofluorescence and the paraformalde-hyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology).
  14. Vikingsson A, Pederson K, Muller D. Enumeration of IFN-gamma producing lymphocytes by flow cytometry and correlation with quantitative measurement of IFN-gamma. J Immunol Methods. 1994; 173(2):219-228. (Biology). 查看参考
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555028 Rev. 1

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