Skip to main content Skip to navigation
CD1a PE
商品详情
Down Arrow Up Arrow


BD™
CD1; T6/Leu-6; R4; T6; HTA-1; FCB6
Human
Mouse IgG2b, κ
Human Thymocytes
Flow cytometry
25 μg/mL
20 μL
909
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


准备和存储

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

333167 Rev. 1
抗体详情
Down Arrow Up Arrow
SK9

CD1a is intended for in vitro diagnostic use in the identification of cells expressing CD1a antigen, using a BD FACS™ brand flow cytometer.

The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate analysis software (such as BD CellQuest™ or BD LYSYS™ II software) for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

333167 Rev. 1
格式详情
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
333167 Rev.1
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "333167" on CiteAb

研发参考 (15)

  1. Boumsell L, Amiot M, Raynal B, Gay-Bellile V, Caillou B, Bernard A. Epitope groups of CD1 molecules. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:289-302.
  2. Ali H, Patry C, Salamero J, et al. Functional and phenotypic analysis of thymic CD34+ CD1a- progenitor-derived dendritic cells: predominance of CD1a- differentiation pathway. J Immunol. 1999; 162:5821. (Biology).
  3. Berger R, Dastugue N, Busson M, et al. t (5; 14)/ HOX11L2–positive T-cell acute lymphoblastic leukemia: a collaborative study of the Groupe Francais Cytogenetique Hematologique (GFCH). Leukemia. 2003; 17:1851-1857. (Biology).
  4. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Clin Cytometry. 2001; 46:23-27. (Biology).
  5. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  6. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. H42-A2. 2007. (Biology).
  7. Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Rothe G, Schmitz G. Leukemia. 1996; 10:877-895. (Biology).
  8. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  9. Ito T, Inaba M, Inaba K, et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.. J Immunol. 1999; 163(3):1409-19. (Biology). 查看参考
  10. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  11. Knowles DM. Knowles DM, Thompson DD, ed. Neoplastic Hematopathology. Philadelphia, PA: Williams & Wilkins; 2001.
  12. Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.. Proc Natl Acad Sci USA. 1987; 84(24):9189-93. (Biology). 查看参考
  13. NCCLS document. 2001. (Biology).
  14. Stelzer GT, Marti G, Hurley A, McCoy PJ, Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997; 30:214-230. (Biology).
  15. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). 查看参考
查看所有文件 (15) 查看更少内容
333167 Rev. 1

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.