BD Pharmingen™ PI/RNase Staining Buffer
Flow cytometric analysis of DNA content of Jurkat cells. Cells from Jurkat cell line (Human T-cell leukemia; ATCC TIB-152) were fixed with 1% paraformaldehyde (methanol free) and stored in 70% ethanol at -20°C. Cells were stained with 0.5 ml of PI/RNase Staining Buffer (Cat. No. 550825) for 15 minutes at room temperature and analyzed by flow cytometry.
Flow cytometric analysis of DNA content of Jurkat cells. Cells from Jurkat cell line (Human T-cell leukemia; ATCC TIB-152) were fixed with 1% paraformaldehyde (methanol free) and stored in 70% ethanol at -20°C. Cells were stained with 0.5 ml of PI/RNase Staining Buffer (Cat. No. 550825) for 15 minutes at room temperature and analyzed by flow cytometry.
Product Details
描述
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, and can reveal useful information about the cell cycle and the proteins involved in cell cycle regulation. Cells in G2 and M phases of the cell cycle have double the DNA content of those in G0 and G1 phases. Cells in S phase have DNA content lying between these extremes. PI is detected in the orange range of the spectrum using a 562-588 nm band pass filter. This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for examining the expression of proteins during the cell cycle.
准备和存储标题文本
推荐的实验流程
Flow cytometry: After fixing and permeabilizing your cell sample, use 0.5 mL /test (1 x 10e6 cells) and incubate for 15 minutes at room temperature before analysis. Please refer to http://static.bdbiosciences.com/documents/BD_FlowCytometry_DNA_Staining_Protocol.pdf for more protocol information.
商品通知
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- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.