BD Phosflow™ Perm/Wash Buffer I

BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins.  BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer.  It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies.  BD Phosflow™ Perm/Wash Buffer I is provided as a  10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer.  Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.