The site is undergoing maintenance. Some pieces of functionality, including sign-in, may be impacted. Ordering can continue through fax and phone. 9:00 a.m HKT on Saturday, May 17, 2025 through 6:00 a.m HKT on Monday, May 19, 2025.
The site is undergoing maintenance. Some pieces of functionality, including sign-in, may be impacted. Ordering can continue through fax and phone.

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The site is undergoing maintenance. Some pieces of functionality, including sign-in, may be impacted. Ordering can continue through fax and phone. 9:00 a.m HKT on Saturday, May 17, 2025 through 6:00 a.m HKT on Monday, May 19, 2025.
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流式细胞仪简介
这些模块将为您介绍流式细胞仪和BD流式细胞仪的原理和基本概念。
课程详情
本课程专为新接触流式细胞仪或期望复习或更新知识的学习者设计。在本课程中,您将学习有关流式细胞仪的关键术语和原理,更好地了解细胞仪子系统及其协作能力。课程还将为您介绍基本的数据分析和分选原则。
每个模块首先介绍主题,结束时会进行一个简短测试,以检查您的知识掌握情况。您可以单独查看每个模块或同时查看所有模块,全面了解流式细胞仪。
流式细胞仪模块简介
概述
概述模块介绍了流式细胞仪的基础知识。查看完成后,您将回答以下问题:
- 什么是流式细胞仪?
- 流式细胞仪适合分析哪种类型的样本?
- 细胞仪可以对哪些细胞特征进行测量?
- 流式细胞仪中的三个子系统是什么?
光学系统
光学系统模块深入探究了激发光学系统和收集光学系统在流式细胞仪中的工作原理。查看完成后,您将回答以下问题:
- 光学系统的主要功能是什么?
- 激光器在BD流式细胞仪中有何作用?
- 长波通滤片、带通滤片和短波通滤片如何将特定光的波长送到适当的检测器上?
光学测量
光学测量模块探索了粒子通过激光束后的光散射情况。查看完成后,您将回答以下问题:
- 影响光散射的因素有哪些?
- 散射光和荧光如何用于检测细胞群?
- 在使用中为什么要知道荧光染料的激发和发射光谱?
- 何为补偿?为什么多色实验中需要补偿?
分选系统
分选系统模块介绍了通过光散射或荧光特性对混合细胞群或微粒进行物理分离的技术。查看完成后,您将回答以下问题:
- 细胞或微粒分选的目的是什么?
- 微粒大小如何影响喷嘴尺寸和鞘液流压力选择?
- 液滴分选的步骤是什么?
BD-59220 (v1.0) 0422