FITC Mouse Anti-Human CD154

BD Pharmingen™ FITC Mouse Anti-Human CD154

Name: FITC Mouse Anti-Human CD154
Brand: FITC Mouse Anti-Human CD154
SKU: 561721

克隆 TRAP1 (also known as TRAP-1) (RUO)

Profile of TPA+Ca++ Ionophore-stimulated PBMC analyzed on a FACScan (BDIS, San Jose, CA)

光谱浏览器

实验方案

科学资源

目录编号:558191

您的价格:登录

目录价格:¥7,280.00

单位:EA (1 Each)

尺寸:

数量:

添加至购物车

保存至购物清单请求散装试剂报价

商品详情

Brand: BD Pharmingen™

Alternative Name: CD40LG; CD40 ligand; CD40L; TNFSF5; T-BAM; TRAP

Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human TRAP1 Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VI 6T-068

RRID: AB_396049

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

数据表

技术数据表（TDS）

配套商品

FITC Mouse IgG1, κ Isotype Control RUO

尺寸 100 Tests 品类编号 555748

最近查看的内容

BD Pharmingen™ PE-Cy™7 Rat Anti-Mouse CD11a(2D7) RUO

品类编号 558191

561721 Rev. 5

抗体详情

TRAP1

The TRAP1 monoclonal antibody specifically binds to CD154. CD154 is a 39 kDa type II membrane glycoprotein that is a member of the tumor necrosis factor superfamily, Tumor necrosis factor ligand superfamily member 5 (TNFSF5). CD154 is expressed on a variety of cell types including activated CD4+ T cells and some CD8+ T cells, NK cells, mast cells and basophils. CD154 is also known as CD40 ligand (CD40L); it serves as a ligand for CD40 that is expressed on B cells, macrophages, and dendritic cells. The expression of CD154 by activated T-helper cells costimulates B-cell activation and proliferation through binding to CD40 expressed on B cells. In response to T-dependent antigens, the CD154 and CD40 interaction is required for B-lymphocyte differentiation, including immunoglobulin production and isotype switching and memory B cell generation. The TRAP1 antibody can partially block T cell-B cell interaction and inhibit the subsequent proliferation, differentiation, and memory formation of B cells. It has been reported that patients with X-linked hyper-IgM syndrome have defective expression of functional CD154 due to mutations in the CD40LG gene that encodes CD154.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

561721 Rev. 5

格式详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: FITC

最多激发源: 494 nm

最多发射源: 518 nm

561721 Rev.5

报价单和参考

View Development References

研发参考 (6)

Fuleihan R, Ramesh N, Horner A, et al. Cyclosporin A inhibits CD40 ligand expression in T lymphocytes. J Clin Invest. 1994; 93(3):1315-1320. (Biology). 查看参考
Gray D, Dullforce P, Jainandunsing S. Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction. J Exp Med. 1994; 180(1):141-155. (Biology). 查看参考
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
Nishioka Y, Lipsky PE. The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration. J Immunol. 1994; 153(3):1027-1036. (Biology). 查看参考
van Kooten C, Gaillard C, Galizzi JP, et al. B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40. Eur J Immunol. 1994; 24(4):787-792. (Biology). 查看参考

查看所有文件 (6)

561721 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.