FITC Goat Anti-Mouse Ig
Clone Polyclonal (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Goat Ig
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
Immunofluorescence (Tested During Development)
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This antibody was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with rat, human, bovine, and horse serum proteins (multiple adsorption).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Flow cytometry: As a second step reagent for staining rat splenocytes, it is reactive with mouse antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. However it has been reported that reactivity with some mouse IgM monoclonal antibodies is weak. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with no non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse antibodies, particularly mouse IgG. For optimal staining of human leukocytes, with mouse IgG and IgM primary antibodies, FITC goat anti-mouse IgG + IgM (human-, bovine-, and horse-adsorbed, Cat. No. 555988) is recommended.