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Purified Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain
商品详情
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BD Pharmingen™
Igl; Ig λ; Immunoglobulin lambda chain complex; Ig λ1/λ2/λ3
Mouse (QC Testing)
Rat IgG2a, κ
Pooled Mouse Ig
ELISA (Routinely Tested)
0.5 mg/ml
111519
AB_394852
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


推荐的实验流程

For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553433) cocktailed with Biotin Rat Anti-Mouse Ig, κ Light Chain (Cat. No. 559750) is optimal for detection with any of the following anti-mouse Ig isotype-specific mAbs: Purified Rat Anti-Mouse IgG1 (Cat. No. 553440); Purified Rat Anti-Mouse IgG2a (Cat. No. 553387); Purified Rat Anti-Mouse IgG2b (Cat. No. 553392); R40-82 [available by custom order]); Purified Rat Anti-Mouse IgM (Cat. No. 553435); Purified Rat Anti-Mouse IgA (Cat. No. 556969); Purified Rat Anti-Mouse IgE (Cat. No. 553413) for capture, respectively.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553432 Rev. 12
抗体详情
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R26-46

The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.

553432 Rev. 12
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553432 Rev.12
报价单和参考
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研发参考 (2)

  1. Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. (Clone-specific). 查看参考
  2. Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. (Clone-specific). 查看参考
553432 Rev. 12

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.