准备和存储
推荐的实验流程
Immunofluorescent staining: For flow cytometric detection of intracytoplasmic IgG1, we recommend the directly conjugated clone, FITC-conjugated mAb A85-1 (Cat. No. 553443).
ELISA: For the sandwich mouse IgG1 ELISA, biotinylated A85-1 mAb (Cat. No. 553441) is optimal for detection with purified anti-mouse IgG1 A85-3 mAb (Cat. No. 553445) for capture.
IHC: For Immunohistochemical staining the Biotinylated clone A85-1 is recommended, Cat. No. 550331
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
