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Reagents
- Flow Cytometry Reagents
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蛋白质印迹试剂
- 免疫分析 试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Analysis of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of a BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
Analysis of Sox2 on human ES cell-derived neural stem cells (NSC). NSC derived from H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
BD Horizon™ V450 Mouse anti-Sox2
BD Horizon™ V450 Mouse anti-Sox2
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准备和存储
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
配套商品
The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
研发参考 (3)
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Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). 查看参考
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Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). 查看参考
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Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). 查看参考
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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