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      Purified Rat Anti-Mouse CD134
      Alert icon
      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      商品详情
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      BD Pharmingen™
      Tnfrsf4; Ox40; OX40L receptor; Ly-70; ACT35; Txgp1; TXGP1L
      Mouse (QC Testing)
      Rat AO IgG1, κ
      Recombinant Mouse OX-40 3/4 CD4 Chimeric Protein
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Western blot (Reported)
      0.5 mg/ml
      AB_397348
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      559861 Rev. 3
      抗体详情
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      OX-86

      The OX-86 monoclonal antibody specifically binds to the OX-40 antigen (CD134), also known as OX-40L receptor. CD134 is a 50-kDa type-I membrane glycoprotein that belongs to the NGFR/TNFR superfamily. Mouse CD134 is expressed on activated CD4+ and CD8+ T lymphocytes and has been shown to be the sole receptor for the OX-40 Ligand (OX-40L). In the brains of mice with actively induced experimental allergic encephalomyelitis, the expression of CD134 on CD4+ T lymphocytes correlates with disease progression. The OX-40/OX-40L system supplies a costimulatory signal for T-cell proliferation and B-cell proliferation and differentiation. In addition, OX-40 antigen provides a costimulatory signal that induces T cells to proliferate in a CD28-independent manner. In the intact animal, CD134 does not appear to be essential for many T-cell responses, but it seems to play a major role in the pathogenesis of some autoimmune diseases. The OX-86 mAb stains both CD4+ and CD8+ activated T cells, and this expression pattern has been confirmed using OX-40L-Ig fusion protein. CD134 was also detected, using OX-86 mAb, on B cells after stimulation with anti-IgM plus anti-CD40 mAb HM40-3 (Cat. no. 553721). OX-86 mAb does not block binding of OX-40L to OX-40, and it stimulates T-cell proliferation mildly.

      559861 Rev. 3
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      559861 Rev.3
      报价单和参考
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      View product citations for antibody "559861" on CiteAb

      研发参考 (8)

      1. Akiba H, Oshima H, Takeda K, et al. CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. J Immunol. 1999; 162(12):7058-7066. (Biology). 查看参考
      2. Baum PR, Gayle RB 3rd, Ramsdell F, et al. Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34. EMBO J. 1994; 13(17):3992-4001. (Biology). 查看参考
      3. Higgins LM, McDonald SA, Whittle N, Crockett N, Shields JG, MacDonald TT. Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein. J Immunol. 1999; 162(1):486-493. (Clone-specific: Western blot). 查看参考
      4. Pippig SD, Pena-Rossi C, Long J, et al. Robust B cell immunity but impaired T cell proliferation in the absence of CD134 (OX40). J Immunol. 1999; 163(12):6520-6529. (Biology). 查看参考
      5. Stuber E, Neurath M, Calderhead D, Fell HP, Strober W. Cross-linking of OX40 ligand, a member of the TNF/NGF cytokine family, induces proliferation and differentiation in murine splenic B cells. Immunity. 1995; 2(5):507-521. (Biology). 查看参考
      6. Weinberg AD, Vella AT, Croft M. OX-40: life beyond the effector T cell stage. Semin Immunol. 1998; 10(6):471-480. (Biology). 查看参考
      7. Weinberg AD, Wegmann KW, Funatake C, Whitham RH. Blocking OX-40/OX-40 ligand interaction in vitro and in vivo leads to decreased T cell function and amelioration of experimental allergic encephalomyelitis. J Immunol. 1999; 162(3):1818-1826. (Biology). 查看参考
      8. al-Shamkhani A, Birkeland ML, Puklavec M, Brown MH, James W, Barclay AN. OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand. Eur J Immunol. 1996; 26(8):1695-1699. (Immunogen). 查看参考
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      559861 Rev. 3

       

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.