Purified NA/LE Rat Anti-Mouse CD62P

BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD62P

Name: Purified NA/LE Rat Anti-Mouse CD62P
Brand: BD Pharmingen™
SKU: 553741

克隆 RB40.34

(RUO)

商品详情

Brand: BD Pharmingen™

Alternative Name: P-selectin; Selp; LECAM3; LYAM3; PADGEM; GMP-140; Grmp

Reactivity: Mouse (QC Testing)

Isotype: Rat LEW, also known as Lewis IgG1, λ

Immunogen: P-selectin-IgG1 Fusion

Application: Flow cytometry (Routinely Tested), Blocking, ELISA, Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation (Reported)

Concentration: 1.0 mg/ml

Entrez Gene ID: 20344

RRID: AB_395023

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Purified NA/LE Rat IgG1, λ Isotype Control RUO

尺寸 0.5 mg 品类编号 559157

FITC Goat Anti-Rat Ig RUO

尺寸 0.5 mg 品类编号 554016

Purified Rat Anti-Mouse CD62P RUO

尺寸 0.5 mg 品类编号 553742

Purified Rat Anti-Mouse CD62P RUO

尺寸 1 mL 品类编号 550289

553741 Rev. 11

抗体详情

RB40.34

The RB40.34 monoclonal antibody specifically binds to mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.

553741 Rev. 11

格式详情

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

格式: NA/LE

553741 Rev.11

报价单和参考

研发参考 (8)

Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Clone-specific: Blocking). 查看参考
Bosse R, Vestweber D. Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum. Eur J Immunol. 1994; 24(12):3019-3024. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation). 查看参考
Hirata T, Furie BC, Furie B. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol. 2002; 169(8):4307-4313. (Clone-specific: Blocking). 查看参考
Katakai T, Mori KJ, Masuda T, Shimizu A. Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis. Int Immunol. 2002; 14(2):167-175. (Clone-specific: Immunohistochemistry). 查看参考
Ley K, Bullard DC, Arbones ML, et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med. 1995; 181(2):669-675. (Clone-specific: Blocking). 查看参考
Pendl GG, Robert C, Steinert M, et al. Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1. Blood. 2002; 99(3):946-956. (Clone-specific: Blocking). 查看参考
Tietz W, Allemand Y, Borges E, et al. CD4+ T cells migrate into inflamed skin only if they express ligands for E- and P-selectin. J Immunol. 1998; 161(2):963-970. (Clone-specific: Blocking). 查看参考
Yang J, Galipeau J, Kozak CA, Furie BC, Furie B. Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. Blood. 1996; 87(10):4176-4186. (Biology). 查看参考

查看所有文件 (8)

553741 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.