PE Rat Anti-Mouse CRIg

BD Pharmingen™ PE Rat Anti-Mouse CRIg

Name: PE Rat Anti-Mouse CRIg
Brand: BD Pharmingen™
SKU: 566737

克隆 17C9

(RUO)

Two-color flow cytometric analysis of CRIg expression on mouse peritoneal macrophages. Resident peritoneal cells were harvested from BALB/c mice, washed, and then stained with BD Horizon™ BUV395 Rat Anti-Mouse F4/80 antibody (Cat. No. 565614) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Middle Plot) or PE Rat Anti-Mouse CRIg antibody (Cat. No. 566737; Right Plot) at 0.5 µg/test. Two-color flow cytometric dot plots showing the correlated expression of CRIg (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable macrophages (Left Plot). Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

光谱浏览器

协议库

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: CRIg; Vsig4; Z39IG

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Mouse CRIg Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 278180

RRID: AB_2869839

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 mL 品类编号 554657

PE Rat IgG2a, κ Isotype Control RUO

尺寸 0.1 mg 品类编号 553930

BUV395 Rat Anti-Mouse F4/80 RUO

尺寸 50 µg 品类编号 565614

566737 Rev. 1

抗体详情

17C9

The 17C9 monoclonal antibody specifically recognizes Complement receptor of the immunoglobulin superfamily (CRIg) which is encoded by the Vsig4 (V-set and immunoglobulin domain containing 4) gene. CRIg is a ~45 kDa single-pass type I transmembrane glycoprotein that contains a variable (V-type) immunoglobulin domain in its extracellular region which is required for binding complement components and promoting phagocytosis. CRIg is expressed on Kupffer cells and tissue resident macrophages, including a subset of resident peritoneal macrophages. CRIg binds complement fragments C3b and iC3b and plays important roles in innate and adaptive immunity. This complement receptor is involved in regulation of the complement cascade and serves as a phagocytic receptor in the clearance of pathogens, immune complexes, and apoptotic cells. CRIg-positive macrophages can reportedly function in the negative regulation of T-cell proliferation and IL-2 production.

566737 Rev. 1

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

566737 Rev.1

报价单和参考

研发参考 (4)

Fu W, Wojtkiewicz G, Weissleder R, Benoist C, Mathis D. Early window of diabetes determinism in NOD mice, dependent on the complement receptor CRIg, identified by noninvasive imaging. Nat Immunol. 2012; 13(4):361-368. (Clone-specific: Flow cytometry). 查看参考
Helmy KY, Katschke KJ, Jr., et al. CRIg: a macrophage complement receptor required for phagocytosis of circulating pathogens. Cell. 2006; 124(5):915-927. (Biology). 查看参考
Small AG, Al-Baghdadi M, Quach A, Hii C, Ferrante A. Complement receptor immunoglobulin: a control point in infection and immunity, inflammation and cancer. Swiss Med Wkly. 2016; 146:w14301. (Biology). 查看参考
Vogt L, Schmitz N, Kurrer MO, et al. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J Clin Invest. 2006; 116(10):2817-2826. (Biology). 查看参考

查看所有文件 (4)

566737 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.